FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017295289> ?p ?o ?g. }

• W2017295289 endingPage "214" @default.
• W2017295289 startingPage "207" @default.
• W2017295289 abstract "Abstract Background: Wide‐field frequency‐domain fluorescence lifetime imaging microscopy (FLIM) is an established technique to determine fluorescence lifetimes. Disadvantage of wide‐field imaging is that measurements are compromised by out‐of‐focus blur. Conventional scanning confocal typically means long acquisition times and more photo bleaching. An alternative is spinning‐disc confocal whereby samples are scanned simultaneously by thousands of pinholes, resulting in a virtually instantaneous image with more than tenfold reduced photo bleaching. Methods: A spinning disc unit was integrated into an existing FLIM system. Measurements were made of fluorescent beads with a lifetime of 2.2 ns against a 5.3 ns fluorescent background outside the focal plane. In addition, living HeLa cells were imaged with different lifetimes in the cytosol and the plasma membrane. Results: In spinning‐disc mode, a lifetime of the beads of 2.8 ns was measured, whereas in wide field a lifetime of 4.1 ns was measured. Lifetime contrast within living HeLa cells could be resolved with the spinning‐disc unit, where this was impossible in wide field. Conclusions: Integration of a spinning‐disc unit into a frequency‐domain FLIM instrument considerably reduces artifacts, while maintaining the advantages of wide field. For FLIM on objects with 3D lifetime structure, spinning‐disc is by far preferable over wide‐field measurements. © 2007 International Society for Analytical Cytology." @default.
• W2017295289 created "2016-06-24" @default.
• W2017295289 creator A5030899768 @default.
• W2017295289 creator A5083451833 @default.
• W2017295289 creator A5085029333 @default.
• W2017295289 creator A5087007179 @default.
• W2017295289 creator A5091618025 @default.
• W2017295289 date "2007-01-31" @default.
• W2017295289 modified "2023-10-17" @default.
• W2017295289 title "Combination of a spinning disc confocal unit with frequency‐domain fluorescence lifetime imaging microscopy" @default.
• W2017295289 cites W1973252991 @default.
• W2017295289 cites W1973740179 @default.
• W2017295289 cites W1979735910 @default.
• W2017295289 cites W1981564484 @default.
• W2017295289 cites W1985102322 @default.
• W2017295289 cites W1989752411 @default.
• W2017295289 cites W1991565661 @default.
• W2017295289 cites W1993272328 @default.
• W2017295289 cites W1998119231 @default.
• W2017295289 cites W2001020189 @default.
• W2017295289 cites W2001385208 @default.
• W2017295289 cites W2003224117 @default.
• W2017295289 cites W2020556902 @default.
• W2017295289 cites W2020828492 @default.
• W2017295289 cites W2025864868 @default.
• W2017295289 cites W2027890668 @default.
• W2017295289 cites W2060714866 @default.
• W2017295289 cites W2068417948 @default.
• W2017295289 cites W2069699841 @default.
• W2017295289 cites W2072199417 @default.
• W2017295289 cites W2100755157 @default.
• W2017295289 cites W2122281782 @default.
• W2017295289 cites W2129995310 @default.
• W2017295289 cites W2147229775 @default.
• W2017295289 cites W2164862869 @default.
• W2017295289 cites W2171196112 @default.
• W2017295289 cites W4245263664 @default.
• W2017295289 doi "https://doi.org/10.1002/cyto.a.20379" @default.
• W2017295289 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17266147" @default.
• W2017295289 hasPublicationYear "2007" @default.
• W2017295289 type Work @default.
• W2017295289 sameAs 2017295289 @default.
• W2017295289 citedByCount "35" @default.
• W2017295289 countsByYear W20172952892012 @default.
• W2017295289 countsByYear W20172952892013 @default.
• W2017295289 countsByYear W20172952892014 @default.
• W2017295289 countsByYear W20172952892015 @default.
• W2017295289 countsByYear W20172952892016 @default.
• W2017295289 countsByYear W20172952892017 @default.
• W2017295289 countsByYear W20172952892019 @default.
• W2017295289 countsByYear W20172952892020 @default.
• W2017295289 countsByYear W20172952892021 @default.
• W2017295289 countsByYear W20172952892022 @default.
• W2017295289 crossrefType "journal-article" @default.
• W2017295289 hasAuthorship W2017295289A5030899768 @default.
• W2017295289 hasAuthorship W2017295289A5083451833 @default.
• W2017295289 hasAuthorship W2017295289A5085029333 @default.
• W2017295289 hasAuthorship W2017295289A5087007179 @default.
• W2017295289 hasAuthorship W2017295289A5091618025 @default.
• W2017295289 hasConcept C120665830 @default.
• W2017295289 hasConcept C121332964 @default.
• W2017295289 hasConcept C136009344 @default.
• W2017295289 hasConcept C147080431 @default.
• W2017295289 hasConcept C150627866 @default.
• W2017295289 hasConcept C154815118 @default.
• W2017295289 hasConcept C159985019 @default.
• W2017295289 hasConcept C192562407 @default.
• W2017295289 hasConcept C2779178360 @default.
• W2017295289 hasConcept C46141821 @default.
• W2017295289 hasConcept C57477423 @default.
• W2017295289 hasConcept C91881484 @default.
• W2017295289 hasConceptScore W2017295289C120665830 @default.
• W2017295289 hasConceptScore W2017295289C121332964 @default.
• W2017295289 hasConceptScore W2017295289C136009344 @default.
• W2017295289 hasConceptScore W2017295289C147080431 @default.
• W2017295289 hasConceptScore W2017295289C150627866 @default.
• W2017295289 hasConceptScore W2017295289C154815118 @default.
• W2017295289 hasConceptScore W2017295289C159985019 @default.
• W2017295289 hasConceptScore W2017295289C192562407 @default.
• W2017295289 hasConceptScore W2017295289C2779178360 @default.
• W2017295289 hasConceptScore W2017295289C46141821 @default.
• W2017295289 hasConceptScore W2017295289C57477423 @default.
• W2017295289 hasConceptScore W2017295289C91881484 @default.
• W2017295289 hasIssue "4" @default.
• W2017295289 hasLocation W20172952891 @default.
• W2017295289 hasLocation W20172952892 @default.
• W2017295289 hasOpenAccess W2017295289 @default.
• W2017295289 hasPrimaryLocation W20172952891 @default.
• W2017295289 hasRelatedWork W1530645078 @default.
• W2017295289 hasRelatedWork W1978803831 @default.
• W2017295289 hasRelatedWork W2008049857 @default.
• W2017295289 hasRelatedWork W2056668469 @default.
• W2017295289 hasRelatedWork W2085535719 @default.
• W2017295289 hasRelatedWork W2105513520 @default.
• W2017295289 hasRelatedWork W2580002495 @default.
• W2017295289 hasRelatedWork W2726111829 @default.
• W2017295289 hasRelatedWork W2999303294 @default.
• W2017295289 hasRelatedWork W4250585504 @default.

• next