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• W2017304814 abstract "It is estimated that between 35% and 74% of all human genes can undergo alternative splicing. Currently, the most efficient methods for large-scale detection of alternative splicing use expressed sequence tags (ESTs) or microarray analysis. As these methods merely sample the transcriptome, splice variants that do not appear in deeply sampled tissues have a low probability of being detected. We present a new method by which we can predict that an internal exon is skipped (namely whether it is a cassette-exon) merely based on its naked genomic sequence and on the sequence of its mouse ortholog. No other data, such as ESTs, are required for the prediction. Using our method, which was experimentally validated, we detected hundreds of novel splice variants that were not detectable using ESTs. We show that a substantial fraction of the splice variants in the human genome could not be identified through current human EST or cDNA data." @default.
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• W2017304814 date "2004-08-01" @default.
• W2017304814 modified "2023-09-27" @default.
• W2017304814 title "A Non-EST-Based Method for Exon-Skipping Prediction" @default.
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• W2017304814 doi "https://doi.org/10.1101/gr.2572604" @default.
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