Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017306452> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2017306452 endingPage "319" @default.
- W2017306452 startingPage "311" @default.
- W2017306452 abstract "A new chromatographic method for the determination of amino acids is proposed. The method is based on the separation of amino acids by means of ion-pair liquid chromatography and post-column derivatization using 1,2-naphthoquinone-4-sulfonate. The analytical column was a Spherisorb ODS 2. Amino acids were separated by an elution gradient with four linear steps based on increasing the concentration of 2-propanol. Two eluents were used to create the gradient profile: eluent A was an aqueous solution of 20 mM H3PO4 + 20 mM H2PO4− + 15 mM dodecyl sulfate and eluent B was a mixture of aqueous (25 mM H3PO4 + 25 mM H2PO4− + 18.5 mM dodecyl sulfate)-2-propanol (1:1, v/v). The injection volume was 100 μl and the total flow-rate for the mobile phase was 0.8 ml/min. The chromatographic outlet was coupled on-line to the two-channel derivatization system which delivered reagent and buffer solutions. The reaction took place at 65°C in a reaction coil of 4 m × 1.1 mm I.D. The spectrophotometric detection was performed at 305 nm. The separation of common amino acids was done in 90 min, although an additional period of 15 min was required to stabilize the column. The repeatability of the method for lysine is 2.1% and the reproducibility is 2.6%. The detection limit for lysine is 0.09 nmol. The linear range for lysine is up to 32 nmol with a correlation coefficient of 0.999. The method was applied to the determination of amino acids in animal feed and powdered milks. The results of the method are in good agreement with those obtained with the standard amino acid autoanalyzer method." @default.
- W2017306452 created "2016-06-24" @default.
- W2017306452 creator A5007994605 @default.
- W2017306452 creator A5017076873 @default.
- W2017306452 date "1994-08-01" @default.
- W2017306452 modified "2023-09-24" @default.
- W2017306452 title "Determination of amino acids by ion-pair liquid chromatography with post-column derivatization using 1,2-naphthoquinone-4-sulfonate" @default.
- W2017306452 cites W1967850027 @default.
- W2017306452 cites W1968095074 @default.
- W2017306452 cites W1971175471 @default.
- W2017306452 cites W1973638061 @default.
- W2017306452 cites W1982285728 @default.
- W2017306452 cites W2006035626 @default.
- W2017306452 cites W2016665723 @default.
- W2017306452 cites W2017470279 @default.
- W2017306452 cites W2028311930 @default.
- W2017306452 cites W2030324734 @default.
- W2017306452 cites W2031307880 @default.
- W2017306452 cites W2043470843 @default.
- W2017306452 cites W2044553926 @default.
- W2017306452 cites W2058354774 @default.
- W2017306452 cites W2059533984 @default.
- W2017306452 cites W2061389031 @default.
- W2017306452 cites W2066899572 @default.
- W2017306452 cites W2068958680 @default.
- W2017306452 cites W2070809734 @default.
- W2017306452 cites W2072852260 @default.
- W2017306452 cites W2079306835 @default.
- W2017306452 cites W2082261438 @default.
- W2017306452 cites W2093487114 @default.
- W2017306452 cites W2094949678 @default.
- W2017306452 cites W57004002 @default.
- W2017306452 cites W2023787713 @default.
- W2017306452 doi "https://doi.org/10.1016/0021-9673(94)80431-1" @default.
- W2017306452 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7921184" @default.
- W2017306452 hasPublicationYear "1994" @default.
- W2017306452 type Work @default.
- W2017306452 sameAs 2017306452 @default.
- W2017306452 citedByCount "51" @default.
- W2017306452 countsByYear W20173064522012 @default.
- W2017306452 countsByYear W20173064522013 @default.
- W2017306452 countsByYear W20173064522014 @default.
- W2017306452 countsByYear W20173064522016 @default.
- W2017306452 countsByYear W20173064522019 @default.
- W2017306452 countsByYear W20173064522020 @default.
- W2017306452 countsByYear W20173064522021 @default.
- W2017306452 countsByYear W20173064522022 @default.
- W2017306452 crossrefType "journal-article" @default.
- W2017306452 hasAuthorship W2017306452A5007994605 @default.
- W2017306452 hasAuthorship W2017306452A5017076873 @default.
- W2017306452 hasConcept C119128265 @default.
- W2017306452 hasConcept C154020017 @default.
- W2017306452 hasConcept C178790620 @default.
- W2017306452 hasConcept C179998833 @default.
- W2017306452 hasConcept C184651966 @default.
- W2017306452 hasConcept C185592680 @default.
- W2017306452 hasConcept C206967267 @default.
- W2017306452 hasConcept C2776923573 @default.
- W2017306452 hasConcept C40875361 @default.
- W2017306452 hasConcept C43617362 @default.
- W2017306452 hasConcept C51724486 @default.
- W2017306452 hasConcept C73826308 @default.
- W2017306452 hasConceptScore W2017306452C119128265 @default.
- W2017306452 hasConceptScore W2017306452C154020017 @default.
- W2017306452 hasConceptScore W2017306452C178790620 @default.
- W2017306452 hasConceptScore W2017306452C179998833 @default.
- W2017306452 hasConceptScore W2017306452C184651966 @default.
- W2017306452 hasConceptScore W2017306452C185592680 @default.
- W2017306452 hasConceptScore W2017306452C206967267 @default.
- W2017306452 hasConceptScore W2017306452C2776923573 @default.
- W2017306452 hasConceptScore W2017306452C40875361 @default.
- W2017306452 hasConceptScore W2017306452C43617362 @default.
- W2017306452 hasConceptScore W2017306452C51724486 @default.
- W2017306452 hasConceptScore W2017306452C73826308 @default.
- W2017306452 hasIssue "2" @default.
- W2017306452 hasLocation W20173064521 @default.
- W2017306452 hasLocation W20173064522 @default.
- W2017306452 hasOpenAccess W2017306452 @default.
- W2017306452 hasPrimaryLocation W20173064521 @default.
- W2017306452 hasRelatedWork W1992834436 @default.
- W2017306452 hasRelatedWork W2001116851 @default.
- W2017306452 hasRelatedWork W2003083611 @default.
- W2017306452 hasRelatedWork W2004191393 @default.
- W2017306452 hasRelatedWork W2021898069 @default.
- W2017306452 hasRelatedWork W2059559280 @default.
- W2017306452 hasRelatedWork W2063401993 @default.
- W2017306452 hasRelatedWork W2084062459 @default.
- W2017306452 hasRelatedWork W2096641431 @default.
- W2017306452 hasRelatedWork W3179568669 @default.
- W2017306452 hasVolume "676" @default.
- W2017306452 isParatext "false" @default.
- W2017306452 isRetracted "false" @default.
- W2017306452 magId "2017306452" @default.
- W2017306452 workType "article" @default.