Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017308641> ?p ?o ?g. }
- W2017308641 abstract "Phosphoglucose isomerase (PGI) catalyzes the interconversion between glucose 6-phosphate and fructose 6-phosphate in the glycolysis pathway. In mammals, the enzyme is also identical to the extracellular proteins neuroleukin, tumor-secreted autocrine motility factor (AMF) and differentiation and maturation mediator for myeloid leukemia. Hereditary deficiency of the enzyme causes non-spherocytic hemolytic anemia in human. In the present study, a novel interaction between GTP and human PGI was corroborated by UV-induced crosslinking, affinity purification and kinetic study. GTP not only inhibits the isomerization activity but also compromises the AMF function of the enzyme. Kinetic studies, including the Yonetani-Theorell method, suggest that GTP is a competitive inhibitor with a Ki value of 63 μM and the GTP-binding site partially overlaps with the catalytic site. In addition, GTP stabilizes the structure of human PGI against heat- and detergent-induced denaturation. Molecular modelling and dynamic simulation suggest that GTP is bound in a syn-conformation with the γ-phosphate group located near the phosphate-binding loop and the ribose moiety positioned away from the active-site residues." @default.
- W2017308641 created "2016-06-24" @default.
- W2017308641 creator A5013992157 @default.
- W2017308641 creator A5031821793 @default.
- W2017308641 creator A5047722243 @default.
- W2017308641 creator A5047871906 @default.
- W2017308641 creator A5059374785 @default.
- W2017308641 creator A5072353570 @default.
- W2017308641 date "2015-07-01" @default.
- W2017308641 modified "2023-09-28" @default.
- W2017308641 title "A novel binding of GTP stabilizes the structure and modulates the activities of human phosphoglucose isomerase/autocrine motility factor" @default.
- W2017308641 cites W1024860189 @default.
- W2017308641 cites W1545450702 @default.
- W2017308641 cites W1556037298 @default.
- W2017308641 cites W1963530308 @default.
- W2017308641 cites W1964260891 @default.
- W2017308641 cites W1965793254 @default.
- W2017308641 cites W1965952267 @default.
- W2017308641 cites W1968406662 @default.
- W2017308641 cites W1981810014 @default.
- W2017308641 cites W1982118513 @default.
- W2017308641 cites W2011406439 @default.
- W2017308641 cites W2017976166 @default.
- W2017308641 cites W2019630682 @default.
- W2017308641 cites W2025793174 @default.
- W2017308641 cites W2032030144 @default.
- W2017308641 cites W2032222439 @default.
- W2017308641 cites W2041791898 @default.
- W2017308641 cites W2045074917 @default.
- W2017308641 cites W2049409997 @default.
- W2017308641 cites W2049686634 @default.
- W2017308641 cites W2051222964 @default.
- W2017308641 cites W2053321729 @default.
- W2017308641 cites W2068948271 @default.
- W2017308641 cites W2069228005 @default.
- W2017308641 cites W2069694136 @default.
- W2017308641 cites W2077004593 @default.
- W2017308641 cites W2084507361 @default.
- W2017308641 cites W2090551173 @default.
- W2017308641 cites W2092972452 @default.
- W2017308641 cites W2095819558 @default.
- W2017308641 cites W2129884882 @default.
- W2017308641 cites W2131009865 @default.
- W2017308641 cites W2134967712 @default.
- W2017308641 cites W2141467202 @default.
- W2017308641 cites W2141725015 @default.
- W2017308641 cites W2156571746 @default.
- W2017308641 cites W2160310346 @default.
- W2017308641 cites W2160544821 @default.
- W2017308641 cites W2286185010 @default.
- W2017308641 cites W2417532218 @default.
- W2017308641 cites W2883755802 @default.
- W2017308641 cites W4210472171 @default.
- W2017308641 doi "https://doi.org/10.1016/j.bbrep.2015.04.003" @default.
- W2017308641 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5668625" @default.
- W2017308641 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29124141" @default.
- W2017308641 hasPublicationYear "2015" @default.
- W2017308641 type Work @default.
- W2017308641 sameAs 2017308641 @default.
- W2017308641 citedByCount "2" @default.
- W2017308641 countsByYear W20173086412018 @default.
- W2017308641 countsByYear W20173086412019 @default.
- W2017308641 crossrefType "journal-article" @default.
- W2017308641 hasAuthorship W2017308641A5013992157 @default.
- W2017308641 hasAuthorship W2017308641A5031821793 @default.
- W2017308641 hasAuthorship W2017308641A5047722243 @default.
- W2017308641 hasAuthorship W2017308641A5047871906 @default.
- W2017308641 hasAuthorship W2017308641A5059374785 @default.
- W2017308641 hasAuthorship W2017308641A5072353570 @default.
- W2017308641 hasBestOaLocation W20173086411 @default.
- W2017308641 hasConcept C118716 @default.
- W2017308641 hasConcept C181199279 @default.
- W2017308641 hasConcept C185592680 @default.
- W2017308641 hasConcept C2781264208 @default.
- W2017308641 hasConcept C55493867 @default.
- W2017308641 hasConcept C88189869 @default.
- W2017308641 hasConceptScore W2017308641C118716 @default.
- W2017308641 hasConceptScore W2017308641C181199279 @default.
- W2017308641 hasConceptScore W2017308641C185592680 @default.
- W2017308641 hasConceptScore W2017308641C2781264208 @default.
- W2017308641 hasConceptScore W2017308641C55493867 @default.
- W2017308641 hasConceptScore W2017308641C88189869 @default.
- W2017308641 hasFunder F4320322795 @default.
- W2017308641 hasLocation W20173086411 @default.
- W2017308641 hasLocation W20173086412 @default.
- W2017308641 hasLocation W20173086413 @default.
- W2017308641 hasLocation W20173086414 @default.
- W2017308641 hasOpenAccess W2017308641 @default.
- W2017308641 hasPrimaryLocation W20173086411 @default.
- W2017308641 hasRelatedWork W1825335475 @default.
- W2017308641 hasRelatedWork W2017308641 @default.
- W2017308641 hasRelatedWork W2044281678 @default.
- W2017308641 hasRelatedWork W2065228811 @default.
- W2017308641 hasRelatedWork W2074542695 @default.
- W2017308641 hasRelatedWork W2080397233 @default.
- W2017308641 hasRelatedWork W2120071368 @default.
- W2017308641 hasRelatedWork W236954974 @default.
- W2017308641 hasRelatedWork W2473477921 @default.
- W2017308641 hasRelatedWork W292816093 @default.
- W2017308641 isParatext "false" @default.