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- W2017309083 abstract "Abstract In order to increase cotton plant resistance to Verticillium dahliae (V. dahliae), an alfalfa antifungal peptide gene (alfAFP), directed by CaMV35S promoter in recombinant binary vector pCAMBIA1301-alf, was delivered into an upland cotton cultivar 5983 (Gossypium hirsutum L.) via an Agrobacterium-mediated hypocotyl system. Molecular analysis confirmed the integration and transcripts of the alfAFP gene in the genome of 12 transformants. In vitro assays showed that crude leaf extracts from transformants was able to significantly (p<0.01) inhibit the growth and proliferation of V. dahliae compared to the extracts from control plants. In vivo assays with fungal pathogens showed that transformants displayed a significant reduction in their disease symptoms compared to control plants. These results suggest that the alfAFP gene was expressed in the upland cotton and its product of gene expression was active in the growth inhibition of V. dahliae." @default.
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- W2017309083 date "2010-01-01" @default.
- W2017309083 modified "2023-09-27" @default.
- W2017309083 title "Expression of alfalfa antifungal peptide gene and enhance of resistance toVerticillium dahliaein upland cotton" @default.
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- W2017309083 doi "https://doi.org/10.1080/09064710802425296" @default.
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