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• W2017334718 abstract "A simple, single tube, hot start, real-time reverse transcription-PCR (rt RT-PCR) technique using SYBR green fluorescence was developed for the detection of genogroup I, cluster 1 Norwalk virus (NV) in stools. Sample dilution and heat release of viral RNA was effective as an alternative to more complex procedures to extract viruses from stool specimens. Real-time RT-PCR was applied to 68 stool isolates from patients participating in a NV volunteer study. First derivative melt curves were used to verify NV amplicon and to rule out the presence of primer dimer or spurious product. A dilution end-point standard curve was developed to semi-quantitate minimum virus levels and the results showed the number of RT-PCR amplifiable NV as high as 6.16 x 10(10)g(-1) of stool. The application of these methods was instrumental in identifying three asymptomatic patients who shed viruses in their stools, thus demonstrating a carrier state among seemingly healthy individuals. This study serves as a model for the development of rapid and specific detection, verification, and quantitation procedures for other Noroviruses in stools." @default.
• W2017334718 created "2016-06-24" @default.
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• W2017334718 date "2004-03-01" @default.
• W2017334718 modified "2023-10-08" @default.
• W2017334718 title "A SYBR green, real-time RT-PCR method to detect and quantitate Norwalk virus in stools" @default.
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• W2017334718 doi "https://doi.org/10.1016/j.jviromet.2003.10.011" @default.
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