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- W2017341808 abstract "The dynamic of hydrophilic photosensitizers was investigated in single cells during light activation. Laser scanning microscopy, steady-state and time-resolved microspectrofluorometry were used to serve the subcellular localization of the drugs and to analyze the fluorescence kinetic during continuous irradiation. The quantitative phototoxic response of the cells was determined at low light doses as well as higher light doses. Morphological changes of the cells as well as phototoxic response were correlated with the fluorescence kinetics of the sensitizers. In detail, meso-tetra porphyrine, tetra-sulfonated aluminum phthalocyanine and Methylene Blue showed a nonlinear kinetic behavior at low light doses which was dependent on the growth phase of the cells. A pycnotic cell nuclei was correlated with a change in the kinetic behavior and observed at higher light doses. In the case of hydrophilic dyes, light induced stimulation of the cells and phototoxic damage are dose dependent and are influenced by the nonlinear kinetics of the photosensitizers." @default.
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- W2017341808 date "1996-11-25" @default.
- W2017341808 modified "2023-09-23" @default.
- W2017341808 title "<title>Nonlinear intracellular dynamics of photosensitizers during PDT</title>" @default.
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- W2017341808 doi "https://doi.org/10.1117/12.260005" @default.
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