Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017346838> ?p ?o ?g. }
- W2017346838 endingPage "148" @default.
- W2017346838 startingPage "139" @default.
- W2017346838 abstract "Phenoloxidase (PO) from the humoral fluid of amphioxus B. belcheri tsingtauense was purified using a sequential combination of ammonium sulphate precipitation, Sephadex G-200 chromatography and DEAE Sepharose™ Fast Flow chromatography. In PAGE, the purified enzyme exhibited a single band of 150 kDa under non-reducing conditions, and was resolved to three bands with molecular masses of 72, 46 and 44 kDa, respectively, under reducing conditions, suggesting that the PO in amphioxus humoral fluid seems to be a heterotrimer of three polypeptides held together by disulphide bonds. The substrate specificity and inhibition characteristics both indicate that the PO isolated from amphioxus humoral fluid is a tyrosinase-type enzyme. In addition, mouse antisera against the purified PO were prepared, and their specificity was confirmed by Western blotting, facilitating the future determination of the origin of PO in the humoral fluid and the distribution of PO-synthesising tissues in amphioxus." @default.
- W2017346838 created "2016-06-24" @default.
- W2017346838 creator A5004629089 @default.
- W2017346838 creator A5008249187 @default.
- W2017346838 creator A5033919240 @default.
- W2017346838 creator A5042348341 @default.
- W2017346838 creator A5091297723 @default.
- W2017346838 date "2005-08-01" @default.
- W2017346838 modified "2023-10-10" @default.
- W2017346838 title "Purification and characterisation of phenoloxidase from amphioxus Branchiostoma belcheri tsingtauense" @default.
- W2017346838 cites W1563009242 @default.
- W2017346838 cites W1589917428 @default.
- W2017346838 cites W168245304 @default.
- W2017346838 cites W1727075458 @default.
- W2017346838 cites W1915026202 @default.
- W2017346838 cites W1940591875 @default.
- W2017346838 cites W1978047479 @default.
- W2017346838 cites W1978864251 @default.
- W2017346838 cites W1985164679 @default.
- W2017346838 cites W1994703148 @default.
- W2017346838 cites W2001137361 @default.
- W2017346838 cites W2002793735 @default.
- W2017346838 cites W2002835408 @default.
- W2017346838 cites W2006010856 @default.
- W2017346838 cites W2006102854 @default.
- W2017346838 cites W2010481365 @default.
- W2017346838 cites W2016078262 @default.
- W2017346838 cites W2016535561 @default.
- W2017346838 cites W2024881274 @default.
- W2017346838 cites W2040276684 @default.
- W2017346838 cites W2044803876 @default.
- W2017346838 cites W2045069280 @default.
- W2017346838 cites W204990578 @default.
- W2017346838 cites W2050241961 @default.
- W2017346838 cites W2051042167 @default.
- W2017346838 cites W2052599394 @default.
- W2017346838 cites W2055957468 @default.
- W2017346838 cites W2063210648 @default.
- W2017346838 cites W2065189343 @default.
- W2017346838 cites W2069183150 @default.
- W2017346838 cites W2078286177 @default.
- W2017346838 cites W2082961872 @default.
- W2017346838 cites W2083293291 @default.
- W2017346838 cites W2085676388 @default.
- W2017346838 cites W2100837269 @default.
- W2017346838 cites W2111301002 @default.
- W2017346838 cites W2116754037 @default.
- W2017346838 cites W2127157137 @default.
- W2017346838 cites W2152263035 @default.
- W2017346838 cites W2167286102 @default.
- W2017346838 cites W2327165416 @default.
- W2017346838 cites W2555099011 @default.
- W2017346838 cites W4253844571 @default.
- W2017346838 cites W4293247451 @default.
- W2017346838 doi "https://doi.org/10.1016/j.fsi.2004.12.001" @default.
- W2017346838 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15752652" @default.
- W2017346838 hasPublicationYear "2005" @default.
- W2017346838 type Work @default.
- W2017346838 sameAs 2017346838 @default.
- W2017346838 citedByCount "25" @default.
- W2017346838 countsByYear W20173468382012 @default.
- W2017346838 countsByYear W20173468382013 @default.
- W2017346838 countsByYear W20173468382014 @default.
- W2017346838 countsByYear W20173468382015 @default.
- W2017346838 countsByYear W20173468382017 @default.
- W2017346838 countsByYear W20173468382019 @default.
- W2017346838 countsByYear W20173468382021 @default.
- W2017346838 crossrefType "journal-article" @default.
- W2017346838 hasAuthorship W2017346838A5004629089 @default.
- W2017346838 hasAuthorship W2017346838A5008249187 @default.
- W2017346838 hasAuthorship W2017346838A5033919240 @default.
- W2017346838 hasAuthorship W2017346838A5042348341 @default.
- W2017346838 hasAuthorship W2017346838A5091297723 @default.
- W2017346838 hasConcept C104317684 @default.
- W2017346838 hasConcept C153911025 @default.
- W2017346838 hasConcept C159654299 @default.
- W2017346838 hasConcept C163994747 @default.
- W2017346838 hasConcept C179247698 @default.
- W2017346838 hasConcept C181199279 @default.
- W2017346838 hasConcept C185592680 @default.
- W2017346838 hasConcept C18903297 @default.
- W2017346838 hasConcept C193363816 @default.
- W2017346838 hasConcept C203014093 @default.
- W2017346838 hasConcept C2776428949 @default.
- W2017346838 hasConcept C2777289219 @default.
- W2017346838 hasConcept C2779881004 @default.
- W2017346838 hasConcept C43617362 @default.
- W2017346838 hasConcept C55493867 @default.
- W2017346838 hasConcept C58121356 @default.
- W2017346838 hasConcept C85265743 @default.
- W2017346838 hasConcept C86803240 @default.
- W2017346838 hasConcept C87554066 @default.
- W2017346838 hasConcept C91523082 @default.
- W2017346838 hasConceptScore W2017346838C104317684 @default.
- W2017346838 hasConceptScore W2017346838C153911025 @default.
- W2017346838 hasConceptScore W2017346838C159654299 @default.
- W2017346838 hasConceptScore W2017346838C163994747 @default.
- W2017346838 hasConceptScore W2017346838C179247698 @default.