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• W2017365073 abstract "Indirect immunofluorescence with rhodamine labelled antibodies and fluoresceinated colchicine (FC) are used to simultaneously localize microtubules and soluble tubulin in cultured ovarian granulosa cells. FC labelled tubulin is most concentrated in regions of the cell occupied by antitubulin stained microtubule bundles. Pretreatment of granulosa cells with colchicine results in a central accumulation of FC and antibody labelled tubulin that coincides with the disposition of 10-nm filament cables. In contrast, the microtubule disrupting agent nocodazole produces a diffuse tubulin distribution as detected with both FC and antibody probes. Taxol treatment, which enhances microtubule assembly, results in a striking concentration of microtubule bundles associated with the nucleus that avidly bind FC. These results suggest that disassembled tubulin is preferentially associated with cytoplasmic microtubules and possibly other formed elements of the cytoskeleton." @default.
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• W2017365073 date "1981-04-01" @default.
• W2017365073 modified "2023-09-27" @default.
• W2017365073 title "Visualization of assembled and disassembled microtubule protein by double label fluorescence microscopy" @default.
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• W2017365073 doi "https://doi.org/10.1016/0309-1651(81)90009-6" @default.
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