Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017367624> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2017367624 endingPage "2467" @default.
- W2017367624 startingPage "2460" @default.
- W2017367624 abstract "Methods of authenticating already canned fish were developed, using polymerase chain reaction (PCR) followed by sequencing and restriction site analysis. The canning process degrades DNA to fewer than 123 base pairs (bp) in length. Therefore, degenerate PCR primers were designed to amplify short (<123 bp) mitochondrial cytochrome b gene sequences known to differ at specific nucleotides among the species of interest. Sequences of canned tuna (Thunnus albacares, Thuunus alalunga, and Katsuwonus pelamis), bonito (Euthynnus affinis), and frigate mackerel (Auxis thazard) were reproducibly identified, and were used to determine which species or whether more than one species was present in individual cans. Restriction site analysis of two amplified regions of the cytochrome b gene demonstrated a faster and less expensive method than sequencing for distinguishing PCR products of different species. Thus, restriction site analysis of PCR products can be used in conjunction with sequencing to authenticate species in canned fish products. Keywords: Polymerase chain reaction; authentication; fish; tuna; bonito; Thunnus; Katsuwonus; Euthynnus; mitochondrial DNA; cytochrome b gene" @default.
- W2017367624 created "2016-06-24" @default.
- W2017367624 creator A5032154733 @default.
- W2017367624 creator A5060287095 @default.
- W2017367624 creator A5081790732 @default.
- W2017367624 date "1996-01-01" @default.
- W2017367624 modified "2023-10-16" @default.
- W2017367624 title "Authentication of Canned Tuna and Bonito by Sequence and Restriction Site Analysis of Polymerase Chain Reaction Products of Mitochondrial DNA" @default.
- W2017367624 cites W2028594199 @default.
- W2017367624 cites W2055043387 @default.
- W2017367624 doi "https://doi.org/10.1021/jf950822t" @default.
- W2017367624 hasPublicationYear "1996" @default.
- W2017367624 type Work @default.
- W2017367624 sameAs 2017367624 @default.
- W2017367624 citedByCount "136" @default.
- W2017367624 countsByYear W20173676242012 @default.
- W2017367624 countsByYear W20173676242013 @default.
- W2017367624 countsByYear W20173676242014 @default.
- W2017367624 countsByYear W20173676242015 @default.
- W2017367624 countsByYear W20173676242016 @default.
- W2017367624 countsByYear W20173676242017 @default.
- W2017367624 countsByYear W20173676242019 @default.
- W2017367624 countsByYear W20173676242020 @default.
- W2017367624 countsByYear W20173676242021 @default.
- W2017367624 countsByYear W20173676242022 @default.
- W2017367624 countsByYear W20173676242023 @default.
- W2017367624 crossrefType "journal-article" @default.
- W2017367624 hasAuthorship W2017367624A5032154733 @default.
- W2017367624 hasAuthorship W2017367624A5060287095 @default.
- W2017367624 hasAuthorship W2017367624A5081790732 @default.
- W2017367624 hasConcept C104317684 @default.
- W2017367624 hasConcept C128319531 @default.
- W2017367624 hasConcept C148292235 @default.
- W2017367624 hasConcept C153911025 @default.
- W2017367624 hasConcept C24586158 @default.
- W2017367624 hasConcept C2778072691 @default.
- W2017367624 hasConcept C2778327988 @default.
- W2017367624 hasConcept C2780532849 @default.
- W2017367624 hasConcept C2780669428 @default.
- W2017367624 hasConcept C2909208804 @default.
- W2017367624 hasConcept C49105822 @default.
- W2017367624 hasConcept C505870484 @default.
- W2017367624 hasConcept C54355233 @default.
- W2017367624 hasConcept C58301748 @default.
- W2017367624 hasConcept C86803240 @default.
- W2017367624 hasConceptScore W2017367624C104317684 @default.
- W2017367624 hasConceptScore W2017367624C128319531 @default.
- W2017367624 hasConceptScore W2017367624C148292235 @default.
- W2017367624 hasConceptScore W2017367624C153911025 @default.
- W2017367624 hasConceptScore W2017367624C24586158 @default.
- W2017367624 hasConceptScore W2017367624C2778072691 @default.
- W2017367624 hasConceptScore W2017367624C2778327988 @default.
- W2017367624 hasConceptScore W2017367624C2780532849 @default.
- W2017367624 hasConceptScore W2017367624C2780669428 @default.
- W2017367624 hasConceptScore W2017367624C2909208804 @default.
- W2017367624 hasConceptScore W2017367624C49105822 @default.
- W2017367624 hasConceptScore W2017367624C505870484 @default.
- W2017367624 hasConceptScore W2017367624C54355233 @default.
- W2017367624 hasConceptScore W2017367624C58301748 @default.
- W2017367624 hasConceptScore W2017367624C86803240 @default.
- W2017367624 hasIssue "8" @default.
- W2017367624 hasLocation W20173676241 @default.
- W2017367624 hasOpenAccess W2017367624 @default.
- W2017367624 hasPrimaryLocation W20173676241 @default.
- W2017367624 hasRelatedWork W1483133128 @default.
- W2017367624 hasRelatedWork W2009820147 @default.
- W2017367624 hasRelatedWork W2017367624 @default.
- W2017367624 hasRelatedWork W2464293920 @default.
- W2017367624 hasRelatedWork W2786869747 @default.
- W2017367624 hasRelatedWork W2910050191 @default.
- W2017367624 hasRelatedWork W4248511571 @default.
- W2017367624 hasRelatedWork W4252937340 @default.
- W2017367624 hasRelatedWork W2184489252 @default.
- W2017367624 hasRelatedWork W2188290306 @default.
- W2017367624 hasVolume "44" @default.
- W2017367624 isParatext "false" @default.
- W2017367624 isRetracted "false" @default.
- W2017367624 magId "2017367624" @default.
- W2017367624 workType "article" @default.