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- W2017368757 abstract "Newly synthesized T7 DNA, formed after infection of Escherichia coli with T7 bacteriophage, is found in long linear molecules predominantly 1, 2 and 3 times the length of the mature molecule. Denaturation of the dimers and trimers and sedimentation of the single chains in alkali reveal unit-length single chains. The fractionation of concatemers broken by shearing on benzoylated, naphthoylated DEAE-cellulose columns reveals that all three length classes adhere to this material by virtue of single-chain regions that are spaced at intervals of about 12 μm, the length of one mature molecule. Annealing experiments with purified “middles” and partially digested mature phage DNA, demonstrate that the single-chain regions are located at the genetic terminals (that is, the XYZABC region) of the monomers and dimers. Studies with an exonuclease, and an endonuclease specific for single chains, reveal that “whiskers” (free-ended single chains) and “gaps” of some sort (a single-chain region having no free ends located in the interior of a double helix) are present. Models that accommodate our findings are shown.It seems likely that these structures are intermediates in the formation of new, mature-sized T7 DNA molecules. A test of likely mutants (those unable to break down concatemeric DNA into mature-size lengths) has turned up no obvious abnormalities in these intermediates.Besides elucidating the structure of T7 intermediates, we have developed techniques which should be generally useful in the study of small single-chain regions of any DNA." @default.
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- W2017368757 date "1967-08-01" @default.
- W2017368757 modified "2023-10-16" @default.
- W2017368757 title "Infectivity of single-stranded rings of bacteriophage lambda DNA" @default.
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- W2017368757 doi "https://doi.org/10.1016/s0022-2836(67)80084-6" @default.
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