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• W2017376402 abstract "ObjectiveThe secreted molecular dialogue of the preimplantation human embryo and the crosstalk with its external environment is a critical factor for reproductive success. The aim of this study was to define the human embryonic protein secretome throughout the preimplantation stage, facilitating a non-invasive method to quantitate embryo developmental potential.DesignIRB approved research study.Materials and MethodsInfertility patients (n=110), donated with consent, spent culture media from 2007-2011 with known outcome. All embryos were cultured in microdrops of sequential media, under oil, to the blastocyst stage. Spent culture media and control microdrops were collected at: (A) day 1 post fertilization, (B) day 3 cleavage stage, and (C) the blastocyst stage. Proteins from culture media samples were separated by 1D gel electrophoresis and analyzed by liquid chromatography mass spectrometry. Proteins were identified using Mascot™ (Matrix Science).ResultsUnique protein secretome profiles reliably distinguished embryos of different developmental stages and implantation potential. In total, 258 proteins were identified in spent culture media, with 74 uniquely characterized in the human embryonic protein secretome. Day 1 spent culture media consisted of 25 unique maternal proteins. All secreted embryonic proteins observed on day 3 (n=16) were also present on day 1 and/or at the blastocyst stage. The blastocyst protein secretome included 54 novel proteins with 33 unique to the blastocyst stage alone. In relation to viable implantation, 12 proteins exhibited increased expression, with known associations to cell signaling.ConclusionThis is the first known study to successfully characterize the human embryonic protein secretome from fertilization to the blastocyst stage. Novel proteins were identified that clearly distinguished a developmentally competent human embryo. In conjunction with morphology, secretome analysis may provide a more comprehensive and enhanced, non-invasive approach for embryo selection in ART. ObjectiveThe secreted molecular dialogue of the preimplantation human embryo and the crosstalk with its external environment is a critical factor for reproductive success. The aim of this study was to define the human embryonic protein secretome throughout the preimplantation stage, facilitating a non-invasive method to quantitate embryo developmental potential. The secreted molecular dialogue of the preimplantation human embryo and the crosstalk with its external environment is a critical factor for reproductive success. The aim of this study was to define the human embryonic protein secretome throughout the preimplantation stage, facilitating a non-invasive method to quantitate embryo developmental potential. DesignIRB approved research study. IRB approved research study. Materials and MethodsInfertility patients (n=110), donated with consent, spent culture media from 2007-2011 with known outcome. All embryos were cultured in microdrops of sequential media, under oil, to the blastocyst stage. Spent culture media and control microdrops were collected at: (A) day 1 post fertilization, (B) day 3 cleavage stage, and (C) the blastocyst stage. Proteins from culture media samples were separated by 1D gel electrophoresis and analyzed by liquid chromatography mass spectrometry. Proteins were identified using Mascot™ (Matrix Science). Infertility patients (n=110), donated with consent, spent culture media from 2007-2011 with known outcome. All embryos were cultured in microdrops of sequential media, under oil, to the blastocyst stage. Spent culture media and control microdrops were collected at: (A) day 1 post fertilization, (B) day 3 cleavage stage, and (C) the blastocyst stage. Proteins from culture media samples were separated by 1D gel electrophoresis and analyzed by liquid chromatography mass spectrometry. Proteins were identified using Mascot™ (Matrix Science). ResultsUnique protein secretome profiles reliably distinguished embryos of different developmental stages and implantation potential. In total, 258 proteins were identified in spent culture media, with 74 uniquely characterized in the human embryonic protein secretome. Day 1 spent culture media consisted of 25 unique maternal proteins. All secreted embryonic proteins observed on day 3 (n=16) were also present on day 1 and/or at the blastocyst stage. The blastocyst protein secretome included 54 novel proteins with 33 unique to the blastocyst stage alone. In relation to viable implantation, 12 proteins exhibited increased expression, with known associations to cell signaling. Unique protein secretome profiles reliably distinguished embryos of different developmental stages and implantation potential. In total, 258 proteins were identified in spent culture media, with 74 uniquely characterized in the human embryonic protein secretome. Day 1 spent culture media consisted of 25 unique maternal proteins. All secreted embryonic proteins observed on day 3 (n=16) were also present on day 1 and/or at the blastocyst stage. The blastocyst protein secretome included 54 novel proteins with 33 unique to the blastocyst stage alone. In relation to viable implantation, 12 proteins exhibited increased expression, with known associations to cell signaling. ConclusionThis is the first known study to successfully characterize the human embryonic protein secretome from fertilization to the blastocyst stage. Novel proteins were identified that clearly distinguished a developmentally competent human embryo. In conjunction with morphology, secretome analysis may provide a more comprehensive and enhanced, non-invasive approach for embryo selection in ART. This is the first known study to successfully characterize the human embryonic protein secretome from fertilization to the blastocyst stage. Novel proteins were identified that clearly distinguished a developmentally competent human embryo. In conjunction with morphology, secretome analysis may provide a more comprehensive and enhanced, non-invasive approach for embryo selection in ART." @default.
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• W2017376402 title "Protein secretome profiles reliably distinguish embryonic stages of development and predict successful IVF outcome" @default.
• W2017376402 doi "https://doi.org/10.1016/j.fertnstert.2012.07.398" @default.
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