FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017385114> ?p ?o ?g. }

• W2017385114 endingPage "314" @default.
• W2017385114 startingPage "305" @default.
• W2017385114 abstract "Autoregulation of the human thyroid hormone receptor beta 1 (hTR beta 1) promoter was assessed by chloramphenicol acetyltransferase and luciferase reporter assays of transient transfections into COS1 and GH3 cells, DNase I footprinting, and gel shift assays. A 5'-deletional analysis of the promoter showed that the region between -906 and -839 and the sequence from -438 to -130 were positively regulated by T3 in COS1 cells cotransfected with an hTR beta 1 expression vector. We also transfected deletion constructs into GH3 cells and showed similar effects of T3 on the trans-activation of the reporters. DNase I footprinting showed a protected inverted palindromic thyroid response element (TRE) at position -890 to -866 in the distal fragment and a direct repeat at position -190 to -166 in the proximal fragment, which were protected by TRs. Mutation of each TRE significantly decreased the trans-activation of the promoter by T3. Gel mobility shift assays showed both proximal and distal TREs formed a retarded band with hTR alpha 1 or hTR beta 1 expressed in COS1 cells and reticulocyte lysates. The bands formed on the distal TRE and the proximal TRE appear to be preferentially formed by a TR homodimer and a heterodimer, respectively. Furthermore, the band formed on the distal TRE disappeared after adding T3 but that on the proximal TRE did not. These results indicate that hTR beta 1 expression is directly regulated by hTR alpha 1, beta 1, and their ligand through two TREs. The different structure of the TREs in this promoter suggests their physiological role in transcriptional regulation may be different." @default.
• W2017385114 created "2016-06-24" @default.
• W2017385114 creator A5030293302 @default.
• W2017385114 creator A5042895299 @default.
• W2017385114 creator A5055811968 @default.
• W2017385114 creator A5069500337 @default.
• W2017385114 creator A5077410853 @default.
• W2017385114 date "1994-03-01" @default.
• W2017385114 modified "2023-09-26" @default.
• W2017385114 title "Two thyroid hormone response elements are present in the promoter of human thyroid hormone receptor beta 1." @default.
• W2017385114 cites W1483740421 @default.
• W2017385114 cites W1565592850 @default.
• W2017385114 cites W1670076014 @default.
• W2017385114 cites W1881800315 @default.
• W2017385114 cites W1968360258 @default.
• W2017385114 cites W1990675136 @default.
• W2017385114 cites W1997031612 @default.
• W2017385114 cites W2011467252 @default.
• W2017385114 cites W2017230913 @default.
• W2017385114 cites W2018442116 @default.
• W2017385114 cites W2041957549 @default.
• W2017385114 cites W2058663633 @default.
• W2017385114 cites W2077580619 @default.
• W2017385114 cites W2090024435 @default.
• W2017385114 cites W2117825435 @default.
• W2017385114 cites W2130929794 @default.
• W2017385114 cites W2131695882 @default.
• W2017385114 cites W2163612441 @default.
• W2017385114 cites W2416455680 @default.
• W2017385114 cites W620778898 @default.
• W2017385114 doi "https://doi.org/10.1210/mend.8.3.8015548" @default.
• W2017385114 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8015548" @default.
• W2017385114 hasPublicationYear "1994" @default.
• W2017385114 type Work @default.
• W2017385114 sameAs 2017385114 @default.
• W2017385114 citedByCount "17" @default.
• W2017385114 countsByYear W20173851142012 @default.
• W2017385114 countsByYear W20173851142016 @default.
• W2017385114 countsByYear W20173851142017 @default.
• W2017385114 crossrefType "journal-article" @default.
• W2017385114 hasAuthorship W2017385114A5030293302 @default.
• W2017385114 hasAuthorship W2017385114A5042895299 @default.
• W2017385114 hasAuthorship W2017385114A5055811968 @default.
• W2017385114 hasAuthorship W2017385114A5069500337 @default.
• W2017385114 hasAuthorship W2017385114A5077410853 @default.
• W2017385114 hasBestOaLocation W20173851141 @default.
• W2017385114 hasConcept C101762097 @default.
• W2017385114 hasConcept C104317684 @default.
• W2017385114 hasConcept C109115496 @default.
• W2017385114 hasConcept C111335760 @default.
• W2017385114 hasConcept C121608353 @default.
• W2017385114 hasConcept C134018914 @default.
• W2017385114 hasConcept C149574134 @default.
• W2017385114 hasConcept C150194340 @default.
• W2017385114 hasConcept C153911025 @default.
• W2017385114 hasConcept C161733203 @default.
• W2017385114 hasConcept C23589133 @default.
• W2017385114 hasConcept C2780730277 @default.
• W2017385114 hasConcept C51445715 @default.
• W2017385114 hasConcept C526584372 @default.
• W2017385114 hasConcept C530470458 @default.
• W2017385114 hasConcept C54009773 @default.
• W2017385114 hasConcept C54355233 @default.
• W2017385114 hasConcept C55493867 @default.
• W2017385114 hasConcept C84606932 @default.
• W2017385114 hasConcept C86803240 @default.
• W2017385114 hasConceptScore W2017385114C101762097 @default.
• W2017385114 hasConceptScore W2017385114C104317684 @default.
• W2017385114 hasConceptScore W2017385114C109115496 @default.
• W2017385114 hasConceptScore W2017385114C111335760 @default.
• W2017385114 hasConceptScore W2017385114C121608353 @default.
• W2017385114 hasConceptScore W2017385114C134018914 @default.
• W2017385114 hasConceptScore W2017385114C149574134 @default.
• W2017385114 hasConceptScore W2017385114C150194340 @default.
• W2017385114 hasConceptScore W2017385114C153911025 @default.
• W2017385114 hasConceptScore W2017385114C161733203 @default.
• W2017385114 hasConceptScore W2017385114C23589133 @default.
• W2017385114 hasConceptScore W2017385114C2780730277 @default.
• W2017385114 hasConceptScore W2017385114C51445715 @default.
• W2017385114 hasConceptScore W2017385114C526584372 @default.
• W2017385114 hasConceptScore W2017385114C530470458 @default.
• W2017385114 hasConceptScore W2017385114C54009773 @default.
• W2017385114 hasConceptScore W2017385114C54355233 @default.
• W2017385114 hasConceptScore W2017385114C55493867 @default.
• W2017385114 hasConceptScore W2017385114C84606932 @default.
• W2017385114 hasConceptScore W2017385114C86803240 @default.
• W2017385114 hasIssue "3" @default.
• W2017385114 hasLocation W20173851141 @default.
• W2017385114 hasLocation W20173851142 @default.
• W2017385114 hasOpenAccess W2017385114 @default.
• W2017385114 hasPrimaryLocation W20173851141 @default.
• W2017385114 hasRelatedWork W2011381989 @default.
• W2017385114 hasRelatedWork W2017385114 @default.
• W2017385114 hasRelatedWork W2029542630 @default.
• W2017385114 hasRelatedWork W2045246106 @default.
• W2017385114 hasRelatedWork W2077063678 @default.
• W2017385114 hasRelatedWork W2091058548 @default.
• W2017385114 hasRelatedWork W2113093767 @default.

• next