FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017385903> ?p ?o ?g. }

• W2017385903 endingPage "1139" @default.
• W2017385903 startingPage "1127" @default.
• W2017385903 abstract "A functional N-type Ca2+ channel (ω-conotoxin GVIA receptor) has been purified from rabbit brain and shown to be composed of four subunits of molecular weights 230 K (α1B), 160 K (α2δ), 95 K and 57 K (β3) [Witcher D. R., De Waard M., Sakamoto J., Franzini-Armstrong C., Pragnell M., Kahl S. D. and Campbell K. D. (1993) Science261: 486–489]. These four subunits migrate on sucrose density gradients as a single complex and are identified by subunit specific polyclonal antibodies. Polyclonal antibodies against the purified receptor complex immunoprecipitate greater than 90% of the [125I]ω-conotoxin GVIA (ω-CgTx) binding sites in solubilized crude rabbit brain membranes. Furthermore, polyclonal antibodies affinity-purified against unique GST fusion proteins from two of the cloned subunits in the complex (α1B and β3) specifically immunoprecipitated [125I]ω-CgTx binding sites and not [3H]PN200-110 binding sites. Analysis of [125I]ω-CgTx binding to the purified N-type Ca2+ channel demonstrated that the equilibrium binding was sensitive to increasing cation concentrations. The IC50 for calcium and barium was 2.5 and 5 mM, respectively. [125II]ω-CgTx binding was not significantly reduced within 15 min after the addition of 50 mM barium. However, single channel analysis of the purified N-type Ca2+ channel preincubated with 10 μM ω-CgTx demonstrated that in the presence of 50 mM barium and 0.5 μM ωCgTx, channel activity was detected but at a low open state probability (P < 0.10). These data suggest that the Ca2+ binding site(s) allosterically regulates the ω-CgTx binding site. Since the channel gating persisted in the presence of ω-CgTx, the ω-CgTx binding site may not be located within the pore of the channel and may be different from intra-pore Ca2+ binding sites." @default.
• W2017385903 created "2016-06-24" @default.
• W2017385903 creator A5027922613 @default.
• W2017385903 creator A5062912509 @default.
• W2017385903 creator A5085822063 @default.
• W2017385903 date "1993-11-01" @default.
• W2017385903 modified "2023-10-18" @default.
• W2017385903 title "Characterization of the purified N-type Ca2+ channel and the cation sensitivity of ω-conotoxin GVIA binding" @default.
• W2017385903 cites W1577883243 @default.
• W2017385903 cites W1777610481 @default.
• W2017385903 cites W1977742032 @default.
• W2017385903 cites W1983423336 @default.
• W2017385903 cites W1984213418 @default.
• W2017385903 cites W1990369193 @default.
• W2017385903 cites W2002199822 @default.
• W2017385903 cites W2006192148 @default.
• W2017385903 cites W2006374456 @default.
• W2017385903 cites W2016788235 @default.
• W2017385903 cites W2022513329 @default.
• W2017385903 cites W2035347592 @default.
• W2017385903 cites W2057664700 @default.
• W2017385903 cites W2065398045 @default.
• W2017385903 cites W2068446924 @default.
• W2017385903 cites W2069775711 @default.
• W2017385903 cites W2082299946 @default.
• W2017385903 cites W2089543949 @default.
• W2017385903 cites W2094482221 @default.
• W2017385903 cites W2098746681 @default.
• W2017385903 cites W2103053020 @default.
• W2017385903 cites W2118309627 @default.
• W2017385903 doi "https://doi.org/10.1016/0028-3908(93)90007-p" @default.
• W2017385903 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8107967" @default.
• W2017385903 hasPublicationYear "1993" @default.
• W2017385903 type Work @default.
• W2017385903 sameAs 2017385903 @default.
• W2017385903 citedByCount "45" @default.
• W2017385903 countsByYear W20173859032012 @default.
• W2017385903 countsByYear W20173859032013 @default.
• W2017385903 countsByYear W20173859032016 @default.
• W2017385903 countsByYear W20173859032018 @default.
• W2017385903 crossrefType "journal-article" @default.
• W2017385903 hasAuthorship W2017385903A5027922613 @default.
• W2017385903 hasAuthorship W2017385903A5062912509 @default.
• W2017385903 hasAuthorship W2017385903A5085822063 @default.
• W2017385903 hasConcept C104292427 @default.
• W2017385903 hasConcept C104317684 @default.
• W2017385903 hasConcept C107824862 @default.
• W2017385903 hasConcept C122229402 @default.
• W2017385903 hasConcept C12554922 @default.
• W2017385903 hasConcept C153911025 @default.
• W2017385903 hasConcept C159654299 @default.
• W2017385903 hasConcept C170493617 @default.
• W2017385903 hasConcept C178790620 @default.
• W2017385903 hasConcept C185592680 @default.
• W2017385903 hasConcept C203014093 @default.
• W2017385903 hasConcept C42086223 @default.
• W2017385903 hasConcept C519063684 @default.
• W2017385903 hasConcept C55493867 @default.
• W2017385903 hasConcept C71240020 @default.
• W2017385903 hasConcept C85520022 @default.
• W2017385903 hasConcept C86803240 @default.
• W2017385903 hasConcept C956191 @default.
• W2017385903 hasConceptScore W2017385903C104292427 @default.
• W2017385903 hasConceptScore W2017385903C104317684 @default.
• W2017385903 hasConceptScore W2017385903C107824862 @default.
• W2017385903 hasConceptScore W2017385903C122229402 @default.
• W2017385903 hasConceptScore W2017385903C12554922 @default.
• W2017385903 hasConceptScore W2017385903C153911025 @default.
• W2017385903 hasConceptScore W2017385903C159654299 @default.
• W2017385903 hasConceptScore W2017385903C170493617 @default.
• W2017385903 hasConceptScore W2017385903C178790620 @default.
• W2017385903 hasConceptScore W2017385903C185592680 @default.
• W2017385903 hasConceptScore W2017385903C203014093 @default.
• W2017385903 hasConceptScore W2017385903C42086223 @default.
• W2017385903 hasConceptScore W2017385903C519063684 @default.
• W2017385903 hasConceptScore W2017385903C55493867 @default.
• W2017385903 hasConceptScore W2017385903C71240020 @default.
• W2017385903 hasConceptScore W2017385903C85520022 @default.
• W2017385903 hasConceptScore W2017385903C86803240 @default.
• W2017385903 hasConceptScore W2017385903C956191 @default.
• W2017385903 hasIssue "11" @default.
• W2017385903 hasLocation W20173859031 @default.
• W2017385903 hasLocation W20173859032 @default.
• W2017385903 hasOpenAccess W2017385903 @default.
• W2017385903 hasPrimaryLocation W20173859031 @default.
• W2017385903 hasRelatedWork W1657973241 @default.
• W2017385903 hasRelatedWork W1968369877 @default.
• W2017385903 hasRelatedWork W2038189702 @default.
• W2017385903 hasRelatedWork W2373874986 @default.
• W2017385903 hasRelatedWork W2382768569 @default.
• W2017385903 hasRelatedWork W2404970265 @default.
• W2017385903 hasRelatedWork W2413892839 @default.
• W2017385903 hasRelatedWork W2995425410 @default.
• W2017385903 hasRelatedWork W3026200651 @default.
• W2017385903 hasRelatedWork W3146540107 @default.
• W2017385903 hasVolume "32" @default.
• W2017385903 isParatext "false" @default.
• W2017385903 isRetracted "false" @default.

• next