FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017393467> ?p ?o ?g. }

• W2017393467 endingPage "119" @default.
• W2017393467 startingPage "104" @default.
• W2017393467 abstract "Virus-like particles (VLPs) are empty particles consisting of virus capsid proteins that closely resemble native virus but are devoid of the native viral nucleic acids and therefore have attracted significant attention as noninfectious vaccines. A recombinant baculovirus, vIBD-7, which encodes the structural proteins (VP2, VP3, and VP4) of infectious bursal disease virus (IBDV), produces native IBD VLPs in infected Spodoptera frugiperda insect cells. Another baculovirus, vEDLH-22, encodes VP2 that is fused with a histidine affinity-tag (VP2H) at the C-terminus. By co-infection with these two baculoviruses, hybrid VLPs with histidine tags were formed and purified by immobilized metal affinity chromatography (Hu et al., 1999). Also, we demonstrated that varying the MOI ratio of these infecting viruses altered the extent of VP2H incorporated into the particles. A dynamic mathematical model that described baculovirus infection and VLP synthesis (Hu and Bentley, 2000) was adapted here for co-infection and validated by immunofluorescence labeling. It was shown to predict the VLP composition as a dynamic function of MOI. A constraint in the VP2H content incorporated into the particles was predicted and shown by experiments. Also, the MOI ratio of both infecting viruses was shown to be the major factor influencing the composition of the hybrid particles and an important factor in determining the overall yield. ELISA results confirmed that VP2H was exhibited to a varied extent on the outer surface of the particles. This model provides insight on the use of virus co-infection in virus-mediated recombinant protein expression systems and aids in the optimization of chimeric VLP synthesis." @default.
• W2017393467 created "2016-06-24" @default.
• W2017393467 creator A5027095675 @default.
• W2017393467 creator A5069619109 @default.
• W2017393467 date "2001-08-13" @default.
• W2017393467 modified "2023-10-09" @default.
• W2017393467 title "Effect of MOI ratio on the composition and yield of chimeric infectious bursal disease virus-like particles by baculovirus co-infection: Deterministic predictions and experimental results" @default.
• W2017393467 cites W1516599231 @default.
• W2017393467 cites W1568396084 @default.
• W2017393467 cites W1573659512 @default.
• W2017393467 cites W1588438024 @default.
• W2017393467 cites W1603847510 @default.
• W2017393467 cites W1745359060 @default.
• W2017393467 cites W1901371944 @default.
• W2017393467 cites W1976468619 @default.
• W2017393467 cites W1982218313 @default.
• W2017393467 cites W1984406334 @default.
• W2017393467 cites W1990381228 @default.
• W2017393467 cites W1996994009 @default.
• W2017393467 cites W1998443530 @default.
• W2017393467 cites W2011509939 @default.
• W2017393467 cites W2025070704 @default.
• W2017393467 cites W2028083923 @default.
• W2017393467 cites W2028167533 @default.
• W2017393467 cites W2031910333 @default.
• W2017393467 cites W2045413107 @default.
• W2017393467 cites W2062293540 @default.
• W2017393467 cites W2068710115 @default.
• W2017393467 cites W2088944980 @default.
• W2017393467 cites W2123047021 @default.
• W2017393467 cites W2130138354 @default.
• W2017393467 cites W2135682000 @default.
• W2017393467 cites W2168591621 @default.
• W2017393467 cites W2172148339 @default.
• W2017393467 cites W2189131273 @default.
• W2017393467 doi "https://doi.org/10.1002/bit.1170" @default.
• W2017393467 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11536133" @default.
• W2017393467 hasPublicationYear "2001" @default.
• W2017393467 type Work @default.
• W2017393467 sameAs 2017393467 @default.
• W2017393467 citedByCount "49" @default.
• W2017393467 countsByYear W20173934672012 @default.
• W2017393467 countsByYear W20173934672013 @default.
• W2017393467 countsByYear W20173934672014 @default.
• W2017393467 countsByYear W20173934672015 @default.
• W2017393467 countsByYear W20173934672016 @default.
• W2017393467 countsByYear W20173934672017 @default.
• W2017393467 countsByYear W20173934672018 @default.
• W2017393467 countsByYear W20173934672020 @default.
• W2017393467 countsByYear W20173934672021 @default.
• W2017393467 countsByYear W20173934672022 @default.
• W2017393467 crossrefType "journal-article" @default.
• W2017393467 hasAuthorship W2017393467A5027095675 @default.
• W2017393467 hasAuthorship W2017393467A5069619109 @default.
• W2017393467 hasConcept C103878061 @default.
• W2017393467 hasConcept C104317684 @default.
• W2017393467 hasConcept C153911025 @default.
• W2017393467 hasConcept C159047783 @default.
• W2017393467 hasConcept C185592680 @default.
• W2017393467 hasConcept C202878990 @default.
• W2017393467 hasConcept C24107716 @default.
• W2017393467 hasConcept C2522874641 @default.
• W2017393467 hasConcept C2776387124 @default.
• W2017393467 hasConcept C2777212814 @default.
• W2017393467 hasConcept C2777773057 @default.
• W2017393467 hasConcept C2777810641 @default.
• W2017393467 hasConcept C40767141 @default.
• W2017393467 hasConcept C55493867 @default.
• W2017393467 hasConcept C60987743 @default.
• W2017393467 hasConcept C86803240 @default.
• W2017393467 hasConcept C89423630 @default.
• W2017393467 hasConceptScore W2017393467C103878061 @default.
• W2017393467 hasConceptScore W2017393467C104317684 @default.
• W2017393467 hasConceptScore W2017393467C153911025 @default.
• W2017393467 hasConceptScore W2017393467C159047783 @default.
• W2017393467 hasConceptScore W2017393467C185592680 @default.
• W2017393467 hasConceptScore W2017393467C202878990 @default.
• W2017393467 hasConceptScore W2017393467C24107716 @default.
• W2017393467 hasConceptScore W2017393467C2522874641 @default.
• W2017393467 hasConceptScore W2017393467C2776387124 @default.
• W2017393467 hasConceptScore W2017393467C2777212814 @default.
• W2017393467 hasConceptScore W2017393467C2777773057 @default.
• W2017393467 hasConceptScore W2017393467C2777810641 @default.
• W2017393467 hasConceptScore W2017393467C40767141 @default.
• W2017393467 hasConceptScore W2017393467C55493867 @default.
• W2017393467 hasConceptScore W2017393467C60987743 @default.
• W2017393467 hasConceptScore W2017393467C86803240 @default.
• W2017393467 hasConceptScore W2017393467C89423630 @default.
• W2017393467 hasIssue "1" @default.
• W2017393467 hasLocation W20173934671 @default.
• W2017393467 hasLocation W20173934672 @default.
• W2017393467 hasOpenAccess W2017393467 @default.
• W2017393467 hasPrimaryLocation W20173934671 @default.
• W2017393467 hasRelatedWork W1740530395 @default.
• W2017393467 hasRelatedWork W2010821699 @default.
• W2017393467 hasRelatedWork W2087306862 @default.
• W2017393467 hasRelatedWork W2150462979 @default.
• W2017393467 hasRelatedWork W2166038746 @default.

• next