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- W2017400811 abstract "ABSTRACT We demonstrated techniques of stepwise changes of poly-ethyleneoxide (PEO) solutions for DNA separations in the presence of the electroosmotic flow (EOF). The stepwise changes were performed by injecting different PEO solutions contained in a number of syringes to a polyethylene tube, from which the polymer solutions entered a capillary filled with 1X TBE buffer by the EOF. The separation of DNA markers V and VI was not complete under isocratic conditions, either using 1.0–2.5% PEO solutions containing 0.5 μg/mL ethidium bromide (EtB) or using 2.0% PEO solutions containing 0.1–2.0 μg/mL EtB. Resolution, sensitivity and time of the DNA separation were varied under conditions of either stepwise changes of PEO concentrations from 1.5 to 2.5% in 0.5 μg/mL EtB solutions or stepwise changes of EtB concentrations from 0.1 to 2.0 μg/mL in 2.0% PEO solutions. The optimal separation was obtained under simultaneous stepwise changes of PEO and EtB: 1.5% PEO containing 0.1μg/mL EtB for 45 s, 1.5% PEO containi..." @default.
- W2017400811 created "2016-06-24" @default.
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- W2017400811 date "2000-11-01" @default.
- W2017400811 modified "2023-09-26" @default.
- W2017400811 title "CAPILLARY ELECTROPHORETIC SEPARATION OF DNA FRAGMENTS UNDER STEPWISE CHANGES OF POLYMER SOLUTIONS" @default.
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- W2017400811 doi "https://doi.org/10.1080/10739140009709735" @default.
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