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- W2017408160 abstract "The plant-infecting mastreviruses (family Geminiviridae) express two distinct replication-initiator proteins, Rep and RepA. Although RepA is essential for systemic infectivity, little is known about its precise function. We therefore investigated its role in replication using 2D-gel electrophoresis to discriminate the replicative forms of Maize streak virus (MSV) mutants that either fail to express RepA (RepA(-)), or express RepA that is unable to bind the plant retinoblastoma related protein, pRBR. Whereas amounts of viral DNA were reduced in two pRBR-binding deficient RepA mutants, their repertoires of replicative forms changed only slightly. While a complete lack of RepA expression was also associated with reduced viral DNA titres, the only traces of replicative intermediates of RepA(-) viruses were those indicative of recombination-dependent replication. We conclude that in MSV, RepA, but not RepA-pRBR binding, is necessary for single-stranded DNA production and efficient rolling circle replication." @default.
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- W2017408160 date "2013-08-01" @default.
- W2017408160 modified "2023-09-30" @default.
- W2017408160 title "Replication modes of Maize streak virus mutants lacking RepA or the RepA–pRBR interaction motif" @default.
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- W2017408160 doi "https://doi.org/10.1016/j.virol.2013.04.012" @default.
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