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- W2017430260 abstract "Cholesterol transfer regulates the intracellular distribution and the metabolism of cholesterol, thus having a direct impact on cholesterol homeostasis in cells. This work investigates the effect of lipid lateral organization on sterol transfer from liposomes to methyl-B-cyclodextrin (MBCD), a water-soluble macrocyclic compound able to deplete sterols from membranes. Large unilamellar vesicles (LUVs) composed of POPC, dehydroergosterol (DHE) and Dansyl-PE were examined. DHE content was varied in steps of 0.4 mol% in a range of concentrations covering the theoretically predicted critical mole fractions (Cr,e.g., 20.0, 22.2, 25.0, 33.3, 40.0 and 50.0 mol%) for maximal sterol superlattice formation. The molar ratio of DHE to Dansyl-PE was kept constant (15:1) in all samples. The rate of sterol transfer was monitored based on the fluorescence resonance energy transfer (FRET) between DHE (donor) and Dansyl-PE (acceptor). When DHE is transferred from LUVs to MBCD, FRET efficiency is decreased and, consequently, the fluorescence intensity of Dansyl-PE is decreased over time. The initial rate of the DHE transfer was found to vary with DHE content in the original membrane in a biphasic manner, reaching a local maximum at all Cr examined. This result demonstrates that the rate of DHE transfer from LUVs to MBCD is governed by the extent of sterol superlattice in the liposomal membrane, a conclusion different from that given in a previous study1. Both studies showed the same general trend, i.e., that sterol chemical potential increases with increasing sterol content. The difference lies in the fine details of how sterol transfer rate varies with sterol content in the immediate vicinity of Cr (AHA, DOD and PDOH). 1 Ali et al. (2007) PNAS 104:5372-7." @default.
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- W2017430260 date "2010-01-01" @default.
- W2017430260 modified "2023-10-18" @default.
- W2017430260 title "Sterol Transfer from Vesicles to MBCD is Governed by the Extent of Sterol Superlattice" @default.
- W2017430260 doi "https://doi.org/10.1016/j.bpj.2009.12.1091" @default.
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