FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017431510> ?p ?o ?g. }

• W2017431510 endingPage "17" @default.
• W2017431510 startingPage "6" @default.
• W2017431510 abstract "Protein 4.2 (P4.2) is an important component in the erythrocyte membrane skeletal network that regulates the stability and flexibility of erythrocytes. Recently, we provided the evidence for specific P4.2 expression in erythroid cells during development (L. Zhu et al., 1998, Blood 91, 695-705). Using dimethyl sulfoxide (DMSO)-induced differentiation of murine erythroleukemia (MEL) cells as a model, transcription of the P4.2 gene was found to be induced during erythroid differentiation. To examine the mechanism for this induction, we isolated the mouse P4.2 genomic DNA containing the 5' flanking sequence and defined the location of the P4.2 promoter. Transcription of the mouse P4.2 gene initiates at multiple sites, with the major initiation site mapped at 174 nucleotides upstream of the ATG start codon. The mouse P4.2 promoter is TATA-less and contains multiple potential binding sites for erythroid transcription factors GATA-1, NF-E2, EKLF, and tal-1/SCL. Transient transfection experiments demonstrated that a 1.7-kb mouse P4.2 promoter fused with the luciferase coding regions was induced in DMSO-treated MEL cells. Deletion analysis showed that a 259-bp P4.2 promoter DNA (nucleotide position -88 to +171 relative to the major transcription initiation site designated +1), containing a GATA-binding site at position -29 to -24, could still respond to the induction in differentiated MEL cells. Importantly, mutations in the -29/-24 GATA motif rendered the promoter unresponsive to DMSO induction. Electrophoretic mobility shift assay revealed that GATA-1 could bind to the -29/-24 GATA motif and this was confirmed by the observation that the nuclear protein bound to the motif was supershifted by an anti-GATA-1 monoclonal antibody. Taken together, these results suggest that the erythroid transcription factor GATA-1 plays an important role in the induction of P4.2 gene expression during erythroid cell differentiation." @default.
• W2017431510 created "2016-06-24" @default.
• W2017431510 creator A5064103438 @default.
• W2017431510 creator A5079571472 @default.
• W2017431510 date "1999-07-01" @default.
• W2017431510 modified "2023-09-25" @default.
• W2017431510 title "Induction of Erythrocyte Protein 4.2 Gene Expression during Differentiation of Murine Erythroleukemia Cells" @default.
• W2017431510 cites W1484108706 @default.
• W2017431510 cites W1505067240 @default.
• W2017431510 cites W1527634600 @default.
• W2017431510 cites W1532617177 @default.
• W2017431510 cites W1536333573 @default.
• W2017431510 cites W1551232709 @default.
• W2017431510 cites W1575638464 @default.
• W2017431510 cites W1577973593 @default.
• W2017431510 cites W1589750045 @default.
• W2017431510 cites W1606472876 @default.
• W2017431510 cites W1626483287 @default.
• W2017431510 cites W1643253926 @default.
• W2017431510 cites W1814301086 @default.
• W2017431510 cites W1968092959 @default.
• W2017431510 cites W1968215478 @default.
• W2017431510 cites W1968891043 @default.
• W2017431510 cites W1969802301 @default.
• W2017431510 cites W1979704489 @default.
• W2017431510 cites W1981460612 @default.
• W2017431510 cites W1983985678 @default.
• W2017431510 cites W1984460767 @default.
• W2017431510 cites W1988755735 @default.
• W2017431510 cites W1990072010 @default.
• W2017431510 cites W1991099584 @default.
• W2017431510 cites W1991646755 @default.
• W2017431510 cites W1994583688 @default.
• W2017431510 cites W1997399778 @default.
• W2017431510 cites W2001772277 @default.
• W2017431510 cites W2014773628 @default.
• W2017431510 cites W2031422316 @default.
• W2017431510 cites W2034943984 @default.
• W2017431510 cites W2037562592 @default.
• W2017431510 cites W2038230521 @default.
• W2017431510 cites W2039373032 @default.
• W2017431510 cites W2040199613 @default.
• W2017431510 cites W2046121370 @default.
• W2017431510 cites W2047389831 @default.
• W2017431510 cites W2049496606 @default.
• W2017431510 cites W2049869441 @default.
• W2017431510 cites W2054338068 @default.
• W2017431510 cites W2055568103 @default.
• W2017431510 cites W2055924980 @default.
• W2017431510 cites W2057830207 @default.
• W2017431510 cites W2070370882 @default.
• W2017431510 cites W2077506429 @default.
• W2017431510 cites W2079513428 @default.
• W2017431510 cites W2082783966 @default.
• W2017431510 cites W2084143397 @default.
• W2017431510 cites W2086548325 @default.
• W2017431510 cites W2087652779 @default.
• W2017431510 cites W2089469841 @default.
• W2017431510 cites W2092385219 @default.
• W2017431510 cites W2094431012 @default.
• W2017431510 cites W2094646101 @default.
• W2017431510 cites W2111470227 @default.
• W2017431510 cites W2112985008 @default.
• W2017431510 cites W2119121495 @default.
• W2017431510 cites W2122079644 @default.
• W2017431510 cites W2122616238 @default.
• W2017431510 cites W2128565119 @default.
• W2017431510 cites W2130130974 @default.
• W2017431510 cites W2138270253 @default.
• W2017431510 cites W2142342476 @default.
• W2017431510 cites W2147951044 @default.
• W2017431510 cites W2151396228 @default.
• W2017431510 cites W2216336415 @default.
• W2017431510 cites W2284120550 @default.
• W2017431510 cites W2293440579 @default.
• W2017431510 cites W2392905741 @default.
• W2017431510 cites W2406838217 @default.
• W2017431510 cites W4233799830 @default.
• W2017431510 cites W4243532900 @default.
• W2017431510 cites W4252346318 @default.
• W2017431510 cites W4255741875 @default.
• W2017431510 cites W586481967 @default.
• W2017431510 cites W68041166 @default.
• W2017431510 cites W1843454879 @default.
• W2017431510 cites W1990528996 @default.
• W2017431510 doi "https://doi.org/10.1006/geno.1999.5846" @default.
• W2017431510 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10395794" @default.
• W2017431510 hasPublicationYear "1999" @default.
• W2017431510 type Work @default.
• W2017431510 sameAs 2017431510 @default.
• W2017431510 citedByCount "10" @default.
• W2017431510 countsByYear W20174315102012 @default.
• W2017431510 countsByYear W20174315102021 @default.
• W2017431510 crossrefType "journal-article" @default.
• W2017431510 hasAuthorship W2017431510A5064103438 @default.
• W2017431510 hasAuthorship W2017431510A5079571472 @default.
• W2017431510 hasConcept C101762097 @default.
• W2017431510 hasConcept C104317684 @default.

• next