Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017441465> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W2017441465 abstract "The Escherichia coli protein RecA catalyzes the strand exchange reaction used in DNA repair and genetic recombination. Previous studies in our lab have shown buffer-specific changes in RecA stability and unfolding transitions. However, these studies suggest only minimal buffer dependent changes in nucleotide binding and secondary structure that did not explain the large buffer dependent differences in RecA stability and unfolding profiles. These observations led to further investigations of how the four common biological buffers Tris, HEPES, MES, and PO4 alter RecA structure and nucleotide binding. Here we have employed circular dichroism (CD) and infrared (IR) spectroscopy to further discern if buffers influence nucleotide binding to RecA. CD spectra of RecA were obtained in the presence and absence of ADP in each buffer condition. Laser-induced photolysis of caged nucleotides was used in conjunction with difference IR to generate RecA-ADP minus RecA difference infrared spectra in each of the four buffers. These studies detected buffer-specific changes in nucleotide binding to RecA including possible perturbations in Gln, Glu, Asp, Asn, Tyr, and Lys residues and unique secondary structural transitions. These differences between RecA-ADP minus RecA difference spectra will be discussed." @default.
- W2017441465 created "2016-06-24" @default.
- W2017441465 creator A5080751465 @default.
- W2017441465 creator A5091061396 @default.
- W2017441465 date "2015-01-01" @default.
- W2017441465 modified "2023-09-29" @default.
- W2017441465 title "Probing Buffer-Specific Effects on Nucleotide Binding to RecA using Difference Ftir" @default.
- W2017441465 doi "https://doi.org/10.1016/j.bpj.2014.11.2815" @default.
- W2017441465 hasPublicationYear "2015" @default.
- W2017441465 type Work @default.
- W2017441465 sameAs 2017441465 @default.
- W2017441465 citedByCount "0" @default.
- W2017441465 crossrefType "journal-article" @default.
- W2017441465 hasAuthorship W2017441465A5080751465 @default.
- W2017441465 hasAuthorship W2017441465A5091061396 @default.
- W2017441465 hasBestOaLocation W20174414651 @default.
- W2017441465 hasConcept C104317684 @default.
- W2017441465 hasConcept C12554922 @default.
- W2017441465 hasConcept C133571119 @default.
- W2017441465 hasConcept C156695909 @default.
- W2017441465 hasConcept C185592680 @default.
- W2017441465 hasConcept C512185932 @default.
- W2017441465 hasConcept C547475151 @default.
- W2017441465 hasConcept C552990157 @default.
- W2017441465 hasConcept C55493867 @default.
- W2017441465 hasConcept C62614982 @default.
- W2017441465 hasConcept C8010536 @default.
- W2017441465 hasConcept C86803240 @default.
- W2017441465 hasConceptScore W2017441465C104317684 @default.
- W2017441465 hasConceptScore W2017441465C12554922 @default.
- W2017441465 hasConceptScore W2017441465C133571119 @default.
- W2017441465 hasConceptScore W2017441465C156695909 @default.
- W2017441465 hasConceptScore W2017441465C185592680 @default.
- W2017441465 hasConceptScore W2017441465C512185932 @default.
- W2017441465 hasConceptScore W2017441465C547475151 @default.
- W2017441465 hasConceptScore W2017441465C552990157 @default.
- W2017441465 hasConceptScore W2017441465C55493867 @default.
- W2017441465 hasConceptScore W2017441465C62614982 @default.
- W2017441465 hasConceptScore W2017441465C8010536 @default.
- W2017441465 hasConceptScore W2017441465C86803240 @default.
- W2017441465 hasLocation W20174414651 @default.
- W2017441465 hasOpenAccess W2017441465 @default.
- W2017441465 hasPrimaryLocation W20174414651 @default.
- W2017441465 hasRelatedWork W1481709637 @default.
- W2017441465 hasRelatedWork W1504086164 @default.
- W2017441465 hasRelatedWork W1504348190 @default.
- W2017441465 hasRelatedWork W1534453690 @default.
- W2017441465 hasRelatedWork W1546428176 @default.
- W2017441465 hasRelatedWork W1582323028 @default.
- W2017441465 hasRelatedWork W1809644669 @default.
- W2017441465 hasRelatedWork W1969913508 @default.
- W2017441465 hasRelatedWork W1973692199 @default.
- W2017441465 hasRelatedWork W1989706823 @default.
- W2017441465 hasRelatedWork W2004406821 @default.
- W2017441465 hasRelatedWork W2005225927 @default.
- W2017441465 hasRelatedWork W2005561489 @default.
- W2017441465 hasRelatedWork W2017059201 @default.
- W2017441465 hasRelatedWork W2017614678 @default.
- W2017441465 hasRelatedWork W2021134177 @default.
- W2017441465 hasRelatedWork W2024007811 @default.
- W2017441465 hasRelatedWork W2030385633 @default.
- W2017441465 hasRelatedWork W2101470643 @default.
- W2017441465 hasRelatedWork W2132943378 @default.
- W2017441465 isParatext "false" @default.
- W2017441465 isRetracted "false" @default.
- W2017441465 magId "2017441465" @default.
- W2017441465 workType "article" @default.