FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017462917> ?p ?o ?g. }

• W2017462917 endingPage "999" @default.
• W2017462917 startingPage "999" @default.
• W2017462917 abstract "A monoclonal antibody specific for Xanthomonas campestris pathovars in the translucens group, including X. c. pv. undulosa, the causal agent of bacterial leaf streak or black chaff in wheat and triticale, was used in immunofluorescence and a dot-immunobinding assay to detect pathogens in aqueous seed extracts. These techniques were compared to dilution plating of seed wash water as potential routine seed-indexing methods. Twenty-six cereal seed lots were harvested from plants with different levels of natural disease incidence grown in three locations in Mexico. Among the 26 lots analyzed, pathogen-free seed came from clean fields principally in Ciudad Obregon, where conditions are less favorable for its development. Pathogen populations detected with dilution plate ranged from 1.3 X 10(4) to 5.3 X 10(6) cfu/g of air-dry weight. Populations were recovered from seed lots that had been stored for 3 yr. Some discrepancies in pathogen detection occurred among the different methods used. Seed lots with high pathogen populations were consistently identified with all three methods. Differences in detection thresholds were found with seed wash water as compared to reference strain from pure culture. Apparently a substantial number of dead cells were present in wash water from some seed lots. Immunofluorescence was more sensitive than dot-immunobinding assay and produced more reproducible results. It is proposed as a standard for indexing germ plasm. Because of the high variation in data, direct counts of fluorescent bacterial cells under UV microscopy will probably not be necessary for identifying infected seed. It is recommended that immunofluorescence-positive seed lots should not be considered for sowing in areas favorable for disease development." @default.
• W2017462917 created "2016-06-24" @default.
• W2017462917 creator A5032711050 @default.
• W2017462917 creator A5038916235 @default.
• W2017462917 date "1992-01-01" @default.
• W2017462917 modified "2023-09-26" @default.
• W2017462917 title "Comparison of Immunofluorescence and Two Assays for Detection of<i>Xanthomonas campestris</i>pv.<i>undulosa</i>in Seeds of Small Grains" @default.
• W2017462917 doi "https://doi.org/10.1094/pd-76-0999" @default.
• W2017462917 hasPublicationYear "1992" @default.
• W2017462917 type Work @default.
• W2017462917 sameAs 2017462917 @default.
• W2017462917 citedByCount "20" @default.
• W2017462917 countsByYear W20174629172017 @default.
• W2017462917 countsByYear W20174629172022 @default.
• W2017462917 crossrefType "journal-article" @default.
• W2017462917 hasAuthorship W2017462917A5032711050 @default.
• W2017462917 hasAuthorship W2017462917A5038916235 @default.
• W2017462917 hasConcept C144027150 @default.
• W2017462917 hasConcept C2780679087 @default.
• W2017462917 hasConcept C2780746887 @default.
• W2017462917 hasConcept C523546767 @default.
• W2017462917 hasConcept C54355233 @default.
• W2017462917 hasConcept C59822182 @default.
• W2017462917 hasConcept C86803240 @default.
• W2017462917 hasConcept C89423630 @default.
• W2017462917 hasConceptScore W2017462917C144027150 @default.
• W2017462917 hasConceptScore W2017462917C2780679087 @default.
• W2017462917 hasConceptScore W2017462917C2780746887 @default.
• W2017462917 hasConceptScore W2017462917C523546767 @default.
• W2017462917 hasConceptScore W2017462917C54355233 @default.
• W2017462917 hasConceptScore W2017462917C59822182 @default.
• W2017462917 hasConceptScore W2017462917C86803240 @default.
• W2017462917 hasConceptScore W2017462917C89423630 @default.
• W2017462917 hasIssue "10" @default.
• W2017462917 hasLocation W20174629171 @default.
• W2017462917 hasOpenAccess W2017462917 @default.
• W2017462917 hasPrimaryLocation W20174629171 @default.
• W2017462917 hasRelatedWork W1660519523 @default.
• W2017462917 hasRelatedWork W2023271816 @default.
• W2017462917 hasRelatedWork W2047134548 @default.
• W2017462917 hasRelatedWork W2047897201 @default.
• W2017462917 hasRelatedWork W2150953285 @default.
• W2017462917 hasRelatedWork W2356543138 @default.
• W2017462917 hasRelatedWork W2400593490 @default.
• W2017462917 hasRelatedWork W3080928308 @default.
• W2017462917 hasRelatedWork W3215139134 @default.
• W2017462917 hasRelatedWork W2461496630 @default.
• W2017462917 hasVolume "76" @default.
• W2017462917 isParatext "false" @default.
• W2017462917 isRetracted "false" @default.
• W2017462917 magId "2017462917" @default.
• W2017462917 workType "article" @default.