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- W2017482634 abstract "2-Desoxy-D-glucose (2DG) has been found to inhibit strongly the anaerobic fermentation of glucose by living yeast. The degree of inhibition was found to be dependent on the molar ratio of substrate to inhibitor, but independent of the absolute quantities of substrate or yeast and of pH of the medium between 4.5 and 7.5.The effect followed the laws of competitive inhibition of an enzyme. The initial rate of fermentation of glucose by fortified cell-free yeast extract was not inhibited by 2DG. The inhibitory effect of 2DG on yeast fermentation was lost with the removal of the yeast cell wall. The evidence indicates that 2DG, acting as a structural analogue of glucose, competitively inhibits the action of a cell-wall enzyme which is essential for the transport of glucose into the cell, thus inhibiting the overall rate of fermentation by living yeast. A new substrate for yeast hexokinase has been discovered through the observation that the adenosine triphosphate-hexokinase system phosphorylated 2-desoxy-D-glucose." @default.
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- W2017482634 title "2-Desoxy-D-glucose as an antagonist of glucose in yeast fermentation" @default.
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- W2017482634 doi "https://doi.org/10.1016/0016-0032(52)90852-1" @default.
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