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• W2017484416 abstract "The human general transcription factor IIF (TFIIF) is required for an accurate transcription initiation by RNA polymerase II and shares some analogous features with the σ subunit of bacterial RNA polymerase. As an attempt to analyze the function of TFIIF, we examined its effect on bacterial transcription in vitro. TFIIF significantly enhanced the initiation of transcription by the bacterial RNA polymerase while other general transcription factors, TATA-binding protein, TFIIB, and TFIIE, did not. The enhancement of the bacterial transcription was ascribed to the 74 kDa subunit of TFIIF (RAP74). RAP74 had an activity of enhancing the binding of the bacterial RNA polymerase to the promoter. The enhancing activity of RAP74 depended on a low molar ratio of the RNA polymerase to the template DNA. The action of RAP74 in the bacterial transcription may be related to a possible regulatory role of RAP74 in the eukaryotic transcription initiation." @default.
• W2017484416 created "2016-06-24" @default.
• W2017484416 creator A5013829353 @default.
• W2017484416 creator A5020971773 @default.
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• W2017484416 date "1994-11-01" @default.
• W2017484416 modified "2023-09-26" @default.
• W2017484416 title "Enhancement of bacterial transcription initiation in vitro by the 74 kDa subunit of human general transcription factor IIF (RAP74)" @default.
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• W2017484416 doi "https://doi.org/10.1016/0167-4781(94)90217-8" @default.
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