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- W2017488295 abstract "Improvements to the polyacrylamide disc electrophoretic technique for the characterization of bacterial dehydrogenases are reported. Enzymes such as lactic dehydrogenase (LDH), succinic dehydrogenase (SDH), malic dehydrogenase (MDH), glutamic dehydrogenase (GDH), and dihydroorotate dehydrogenase (DHOD) were solubilized by treating the bacterial extract with 1.0% Triton X-100. To stabilize the proteins in the disaggregated state the sample and stacking gels contained 0.1% Triton X-100 and the separating gel contained 0.05% Triton X-100. Location of the specific enzymes in the gels was by coupled reduction of p-nitro blue tetrazolium. By using 0.1% Triton X-100 in place of KCN as an inhibitor of oxidase activity and also by incorporating 25 μg MnCl2/ml in the developing solution, gels without nonspecific background diformazan deposit were obtained." @default.
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- W2017488295 title "Improved method for characterization of bacterial dehydrogenases using acrylamide gel disc electrophoresis" @default.
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- W2017488295 doi "https://doi.org/10.1016/0003-2697(70)90387-8" @default.
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