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- W2017496145 abstract "Abstract Time-of-flight correlation methods have been used to determine the primary structure of the major component in a nonstandard preparation of alamethicins, and to give some sequence information about minor components. The peptide (MW ≈ 2000 u) is blocked at the N terminus with an acetyl group and has a primary alcohol rather than a carboxyl group at the C terminus, so the usual wet chemical sequencing methods cannot be applied. Upon bombardment with 25 keVI− ions, the peptide, deposited on the surface of a solid target, produces both molecular ions and prompt fragment ions (i.e. ions formed at or very near the surface of the target). After acceleration, these ions may undergo metastable decay as they pass along the flight tube of a reflecting time-of-flight mass spectrometer. Measurement of the correlations between the neutral and charged daughters from these decompositions determines the decay pattern of each ion, which in turn yields definitive information about the sequence of the original peptide. All events are recorded on magnetic tape and analyzed off-line, so a single run on the spectrometer provides information on the decay of every ion produced at the target, i.e. information similar to that obtainable from a complete set of daughter ion scans on a multiple sector or triple quadrupole instrument." @default.
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- W2017496145 title "Structural measurements on several alamethicin peptides by the time-of-flight correlation technique" @default.
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- W2017496145 doi "https://doi.org/10.1016/0168-1176(94)04116-o" @default.
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