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- W2017564354 abstract "A splice form of prochymosin lacking exon‐6 was subcloned and expressed. Expression was optimized by changing various parameters. The maximum amount of protein expressed by this method was 44.61% of total cellular protein, compared to the 45.4% predicted by the Qualitek‐4 software. The expressed protein was then purified using ion exchange chromatography. Activation of the recombinant prochymosin was carried out by changing different parameters. Optimal conditions for activation involved reduction of pH to 5 and 4 h of incubation with the acidic solution, followed by neutralization and 2 h of incubation under neutralized conditions. The activity of the subsequent enzyme sample was 26 units/mL." @default.
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- W2017564354 date "2009-05-01" @default.
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- W2017564354 title "Optimization of conditions for expression and activation of a splice variant of prochymosin lacking exon 6 inEscherichia coli" @default.
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- W2017564354 doi "https://doi.org/10.1111/j.1471-0307.2009.00474.x" @default.
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