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• W2017568935 abstract "The total nuclear proteins (30% by nuclear dry weight) of the plasmodial slime mould Physarum polycephalum have been quantitatively separated into four distinct solubility classes possessing characteristic molecular weight ranges and electrophoretic profiles. The residual acidic proteins of nuclei and nucleoli have been extracted during synchronous growth and during two forms of differentiation and separated into two fractions with buffer-saturated phenol, pH 8.2, and hot sodium dodecyl sulfate (SDS). Quantitative comparisons of electrophoretically separated proteins of both residual fractions from nucleoli and nuclei reveal numerous changes in the phenol-soluble phosphoproteins during both forms of starvation-induced differentiation, and most of the changes occur in the chromatin-associated proteins ranging in molecular weight from 32,000 to 160,000. Reversion of the changes induced by starvation is complete within 8–10 hr after refeeding and precedes the first post-feeding mitotic division by 2 hr. Only two nucleolar proteins with molecular weights of 37,000 and 41,000 are extractable with phenol and are unchanged during differentiation. Five additional residual nucleolar proteins remaining after phenol extraction can be extracted with hot SDS and one of these proteins with a molecular weight of 86,500 disappears during differentiation. Incorporation, studies with 14C labeled amino acids demonstrate that the new proteins which appear during differentiation are newly synthesized, that proteins which disappear during differentiation incorporate no label during development of the new cell states, and that there are two periods (mid S and late G2) of increased isotope incorporation during active growth. The residual nucleolar proteins as compared to the residual nuclear proteins are high in serine, glycine, and aspartic acid but low in valine and isoleucine." @default.
• W2017568935 created "2016-06-24" @default.
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• W2017568935 date "1973-03-01" @default.
• W2017568935 modified "2023-09-26" @default.
• W2017568935 title "Localization of nucleolar and chromatin residual acidic protein changes during differentiation in Physarum polycephalum" @default.
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• W2017568935 doi "https://doi.org/10.1016/s0003-9861(73)80017-7" @default.
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