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• W2017603944 abstract "The decolorization/degradation of aqueous azo dyes generated by textile photography industries are a main issue in wastewater treatment. Despite many significant efforts and numerous systems have been exploited, the problem is still unsolved due to intrinsically low activity or use of non-convenient chemical reagents. Biodegradation appears to be one of the most efficient ways, however, the initial step of biotransformation usually involves a reductive cleavage of the azo bond, which under anaerobic conditions leads to the formation of aromatic amines known to be carcinogenic. This study shows that applying an oxidative process of H2O2 catalyzed by chloroperoxidase (CPO) for decolorization/degradation of aqueous azo dyes (Orange G and Sunset Yellow) in buffer solution is a very efficient system. The effect of initial pH, dosage of enzyme, H2O2 concentration, reaction temperature and time, and suitable initial dye concentration on the decolorization efficiency were investigated in detail and optimized accordingly. At optimum reaction condition, the decolorization efficiency of Orange G reached 98.72% in 5 min, and 77.25% in 10 min for Sunset Yellow. The dosage of CPO was below ppm level. This enzymatic reaction is an ideal potential alternative for application on decolorization/degradation of aqueous azo dyes. Furthermore, the elucidation of degradation pathways is of special interest considering healthy and environmental priorities. In this work, LC–MS was used to determine the structures of intermediates arising from Orange G by CPO–H2O2 oxidation. Eight transformation products were identified and two types of cleavage were proposed for the degradation pathway." @default.
• W2017603944 created "2016-06-24" @default.
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• W2017603944 date "2012-05-01" @default.
• W2017603944 modified "2023-09-22" @default.
• W2017603944 title "Efficient decolorization/degradation of aqueous azo dyes using buffered H2O2 oxidation catalyzed by a dosage below ppm level of chloroperoxidase" @default.
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• W2017603944 doi "https://doi.org/10.1016/j.cej.2012.03.009" @default.
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