Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017606924> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W2017606924 endingPage "836" @default.
- W2017606924 startingPage "829" @default.
- W2017606924 abstract "Since the invention of PCR, many adaptation techniques have been developed for sequencing DNA fragments flanking known sequences. Of them, inverse PCR is a matter of interest because of the simplicity of its principle. However, the protocols for inverse PCR introduced so far consist of some time-consuming procedures, and with them, we cannot walk chromosomes too far since the number of suitable restriction enzymes is limited. Our experiments led to confirming simpler technical approaches applicable to the case of bacterial chromosomes, that is, designing two end-specific contextual sequences with which we can quickly detect the desired clones of targeted DNA fragments by simply analyzing PCR products, employing the minimum value of the desired fragments as a discriminating minimum value to decrease contaminant DNA fragments, and creating a new tandem of two cleaved end fragments of a known sequence (reordering) for PCR amplification in combination with cloning of the inverse PCR-generated DNA. With the improvements, we could both simplify the procedures and broaden the capacity of the inverse PCR in walking chromosomes." @default.
- W2017606924 created "2016-06-24" @default.
- W2017606924 creator A5007679513 @default.
- W2017606924 creator A5052964378 @default.
- W2017606924 creator A5088907088 @default.
- W2017606924 date "1999-09-01" @default.
- W2017606924 modified "2023-10-16" @default.
- W2017606924 title "Methods for Rapid Cloning and Detection for Sequencing of Cloned Inverse PCR-Generated DNA Fragments Adjacent to Known Sequences in Bacterial Chromosomes" @default.
- W2017606924 cites W1522872684 @default.
- W2017606924 cites W1984915289 @default.
- W2017606924 cites W2008282869 @default.
- W2017606924 cites W2013803304 @default.
- W2017606924 cites W2014669590 @default.
- W2017606924 cites W2019410656 @default.
- W2017606924 cites W2021608281 @default.
- W2017606924 cites W2021673452 @default.
- W2017606924 cites W2032118018 @default.
- W2017606924 cites W2050717506 @default.
- W2017606924 cites W2054565940 @default.
- W2017606924 cites W2063450941 @default.
- W2017606924 cites W2072789260 @default.
- W2017606924 cites W2078788276 @default.
- W2017606924 cites W2108388593 @default.
- W2017606924 cites W2128750289 @default.
- W2017606924 cites W2139767135 @default.
- W2017606924 cites W2144515939 @default.
- W2017606924 cites W2148312059 @default.
- W2017606924 cites W2153277457 @default.
- W2017606924 cites W2211631152 @default.
- W2017606924 cites W2735566229 @default.
- W2017606924 doi "https://doi.org/10.1111/j.1348-0421.1999.tb01217.x" @default.
- W2017606924 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10553675" @default.
- W2017606924 hasPublicationYear "1999" @default.
- W2017606924 type Work @default.
- W2017606924 sameAs 2017606924 @default.
- W2017606924 citedByCount "5" @default.
- W2017606924 crossrefType "journal-article" @default.
- W2017606924 hasAuthorship W2017606924A5007679513 @default.
- W2017606924 hasAuthorship W2017606924A5052964378 @default.
- W2017606924 hasAuthorship W2017606924A5088907088 @default.
- W2017606924 hasBestOaLocation W20176069241 @default.
- W2017606924 hasConcept C104317684 @default.
- W2017606924 hasConcept C121050878 @default.
- W2017606924 hasConcept C153911025 @default.
- W2017606924 hasConcept C199360897 @default.
- W2017606924 hasConcept C20682886 @default.
- W2017606924 hasConcept C41008148 @default.
- W2017606924 hasConcept C49105822 @default.
- W2017606924 hasConcept C51679486 @default.
- W2017606924 hasConcept C54355233 @default.
- W2017606924 hasConcept C552990157 @default.
- W2017606924 hasConcept C70721500 @default.
- W2017606924 hasConcept C86803240 @default.
- W2017606924 hasConcept C90583042 @default.
- W2017606924 hasConceptScore W2017606924C104317684 @default.
- W2017606924 hasConceptScore W2017606924C121050878 @default.
- W2017606924 hasConceptScore W2017606924C153911025 @default.
- W2017606924 hasConceptScore W2017606924C199360897 @default.
- W2017606924 hasConceptScore W2017606924C20682886 @default.
- W2017606924 hasConceptScore W2017606924C41008148 @default.
- W2017606924 hasConceptScore W2017606924C49105822 @default.
- W2017606924 hasConceptScore W2017606924C51679486 @default.
- W2017606924 hasConceptScore W2017606924C54355233 @default.
- W2017606924 hasConceptScore W2017606924C552990157 @default.
- W2017606924 hasConceptScore W2017606924C70721500 @default.
- W2017606924 hasConceptScore W2017606924C86803240 @default.
- W2017606924 hasConceptScore W2017606924C90583042 @default.
- W2017606924 hasIssue "9" @default.
- W2017606924 hasLocation W20176069241 @default.
- W2017606924 hasLocation W20176069242 @default.
- W2017606924 hasOpenAccess W2017606924 @default.
- W2017606924 hasPrimaryLocation W20176069241 @default.
- W2017606924 hasRelatedWork W1995477860 @default.
- W2017606924 hasRelatedWork W2010728624 @default.
- W2017606924 hasRelatedWork W2040162313 @default.
- W2017606924 hasRelatedWork W2052192891 @default.
- W2017606924 hasRelatedWork W2073168434 @default.
- W2017606924 hasRelatedWork W2075632018 @default.
- W2017606924 hasRelatedWork W2076444477 @default.
- W2017606924 hasRelatedWork W2141174609 @default.
- W2017606924 hasRelatedWork W2415645014 @default.
- W2017606924 hasRelatedWork W2954031593 @default.
- W2017606924 hasVolume "43" @default.
- W2017606924 isParatext "false" @default.
- W2017606924 isRetracted "false" @default.
- W2017606924 magId "2017606924" @default.
- W2017606924 workType "article" @default.