FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017641016> ?p ?o ?g. }

• W2017641016 endingPage "3568" @default.
• W2017641016 startingPage "3568" @default.
• W2017641016 abstract "purpose. To determine the role of eukaryotic translation initiation factor 5A (eIF5A) in TNF-α–induced apoptosis of lamina cribrosa (LC) cells. methods. LC cells were isolated from optic nerve heads of eyes of two human donors. The cells were treated with TNF-α and camptothecin, a TNF synergist, and the incidence of apoptosis was scored by Hoechst staining. Expression of eIF5A protein in response to camptothecin or a combination of camptothecin and TNF-α was determined by Western blot analysis. The ability of small inhibitory (si)RNAs directed against eIF5A to protect LC cells from TNF-α–induced apoptosis was determined by Hoechst and TUNEL staining of transfected LC cells. results. TNF-α and camptothecin synergized to induce greater than two times more apoptosis in LC cells than when the cells were treated with TNF-α or camptothecin separately. Expression of eIF5A protein increased significantly after 8 hours of exposure to TNF-α and camptothecin, but not in response to camptothecin alone. siRNAs directed against eIF5A reduced apoptosis of LC cells in response to TNF-α and camptothecin by between 35% and 69%, as determined by Hoechst staining. An siRNA against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) also reduced apoptosis of LC cells by 42%. TUNEL of transfected LC cells treated with TNF-α and camptothecin revealed an 80% reduction in apoptosis with siRNA against eIF5A. conclusions. TNF-α, in synergy with camptothecin, induces apoptosis in human LC cells. eIF5A is upregulated by LC cells in response to TNF-α, and siRNAs against eIF5A protect LC cells from apoptosis. Thus, eIF5A appears to be a novel proapoptotic protein in the TNF pathway and a possible target for treatment of glaucoma." @default.
• W2017641016 created "2016-06-24" @default.
• W2017641016 creator A5018258250 @default.
• W2017641016 creator A5032964753 @default.
• W2017641016 creator A5039384913 @default.
• W2017641016 creator A5054351395 @default.
• W2017641016 creator A5057855111 @default.
• W2017641016 creator A5072479467 @default.
• W2017641016 creator A5080629613 @default.
• W2017641016 creator A5086795169 @default.
• W2017641016 date "2004-10-01" @default.
• W2017641016 modified "2023-09-29" @default.
• W2017641016 title "Role of eIF5A in TNF-α–Mediated Apoptosis of Lamina Cribrosa Cells" @default.
• W2017641016 cites W1171331324 @default.
• W2017641016 cites W1483046909 @default.
• W2017641016 cites W1488039887 @default.
• W2017641016 cites W1493533123 @default.
• W2017641016 cites W1509884519 @default.
• W2017641016 cites W1544068656 @default.
• W2017641016 cites W1544786964 @default.
• W2017641016 cites W1586303833 @default.
• W2017641016 cites W1590682977 @default.
• W2017641016 cites W1624269173 @default.
• W2017641016 cites W1963749512 @default.
• W2017641016 cites W1966157673 @default.
• W2017641016 cites W1970483076 @default.
• W2017641016 cites W1974342849 @default.
• W2017641016 cites W1977730659 @default.
• W2017641016 cites W1980655887 @default.
• W2017641016 cites W1984401984 @default.
• W2017641016 cites W1985542518 @default.
• W2017641016 cites W1987590420 @default.
• W2017641016 cites W1994031750 @default.
• W2017641016 cites W1994214260 @default.
• W2017641016 cites W1994331402 @default.
• W2017641016 cites W1995882746 @default.
• W2017641016 cites W1998871738 @default.
• W2017641016 cites W200153024 @default.
• W2017641016 cites W2002507218 @default.
• W2017641016 cites W2006836578 @default.
• W2017641016 cites W2008944418 @default.
• W2017641016 cites W2014272469 @default.
• W2017641016 cites W2016444344 @default.
• W2017641016 cites W2016728080 @default.
• W2017641016 cites W2019132331 @default.
• W2017641016 cites W2019958880 @default.
• W2017641016 cites W2020793434 @default.
• W2017641016 cites W2030503422 @default.
• W2017641016 cites W2041593752 @default.
• W2017641016 cites W2042502679 @default.
• W2017641016 cites W2043501837 @default.
• W2017641016 cites W2044614437 @default.
• W2017641016 cites W2052878943 @default.
• W2017641016 cites W2059407857 @default.
• W2017641016 cites W2059925177 @default.
• W2017641016 cites W2061000633 @default.
• W2017641016 cites W2066752265 @default.
• W2017641016 cites W2069151409 @default.
• W2017641016 cites W2072465036 @default.
• W2017641016 cites W2074709811 @default.
• W2017641016 cites W2074823714 @default.
• W2017641016 cites W2076339827 @default.
• W2017641016 cites W2077243943 @default.
• W2017641016 cites W2083058646 @default.
• W2017641016 cites W2084352924 @default.
• W2017641016 cites W2088317037 @default.
• W2017641016 cites W2089684638 @default.
• W2017641016 cites W2095013915 @default.
• W2017641016 cites W2105830374 @default.
• W2017641016 cites W2120866442 @default.
• W2017641016 cites W2126610379 @default.
• W2017641016 cites W2134769368 @default.
• W2017641016 cites W2143105805 @default.
• W2017641016 cites W2143331398 @default.
• W2017641016 cites W2150055598 @default.
• W2017641016 cites W2161924983 @default.
• W2017641016 cites W2163925135 @default.
• W2017641016 cites W2163965868 @default.
• W2017641016 cites W2167465526 @default.
• W2017641016 cites W2170021673 @default.
• W2017641016 cites W2414249909 @default.
• W2017641016 cites W2415499460 @default.
• W2017641016 cites W2883780929 @default.
• W2017641016 cites W303348942 @default.
• W2017641016 cites W437518484 @default.
• W2017641016 cites W575273047 @default.
• W2017641016 cites W60487933 @default.
• W2017641016 cites W8233037 @default.
• W2017641016 doi "https://doi.org/10.1167/iovs.03-1367" @default.
• W2017641016 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15452064" @default.
• W2017641016 hasPublicationYear "2004" @default.
• W2017641016 type Work @default.
• W2017641016 sameAs 2017641016 @default.
• W2017641016 citedByCount "34" @default.
• W2017641016 countsByYear W20176410162012 @default.
• W2017641016 countsByYear W20176410162013 @default.
• W2017641016 countsByYear W20176410162014 @default.
• W2017641016 countsByYear W20176410162015 @default.

• next