Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017649369> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2017649369 endingPage "154" @default.
- W2017649369 startingPage "154" @default.
- W2017649369 abstract "Nuclear transfer (NT) is one of the most advanced technologies to increase animal efficiency in livestock production. Re-cloning can be utilized to investigate more effective methods for agricultural, biological, and medical research. However, few studies have been undertaken on re-cloning from cloned animals. The present study was conducted to examine some factors affecting in vitro development of re-cloned embryos and pregnancy by somatic cell nuclear transfer (SCNT). Ear fibroblast cells as karyoplast donors were isolated from a cloned Korean goat, Jinsoonny, 3 weeks after birth and cultured in serum-starvation condition (TCM-199 + 0.5% FBS) for cell confluence. Recipient oocytes were surgically collected by flushing the oviducts at 35 h after hCG injection from FSH-stimulated goats. The zonae pellucidae of the oocytes were partially drilled using a laser system and each somatic cell was individually transferred into an enucleated oocyte. The couplets were electrically fused and activated by ionomycin (5 min) and 6-DMAP (4 h). The reconstructed embryos were cultured in mSOF medium containing 0.8% BSA at 39�C in an atmosphere of 5% CO2, 5% O2, 90% N2 for 12 to 15 h. Re-cloned embryos (2- to 4-cell stages) were surgically transferred into the oviducts of the recipients. Estrous synchronization was induced by 10 days of treatment with a CIDR and a single injection of PGF2�. Pregnancy was diagnosed by progesterone assay and ultrasound on Days 21 and 63 of pregnancy. The fusion and cleavage rates of re-cloned oocytes were 87.5% (182/208) and 56.0% (102/182), respectively. A total of 175 re-cloned embryos were transferred into 28 recipients. Eleven (39.3%) and 4 recipients (14.3%) were confirmed pregnant on Days 21 and 60, respectively. In comparison of pregnany rate by estrous synchronization, a total of 66 and 109 re-cloned embryos were transferred into 11 recipients in natural estrus and 17 recipients in induced estrus, respectively. Five (45.4%) and 2 recipients (18.2%) in natural estrus were confirmed pregnant on Days 21 and 63, while 6 (35.3%) and 2 (11.8%) recipients in induced estrus were pregnant, respectively. There were no differences in pregnancy rate when the recipients were in estrus within 12 h of the donors (40 to 60%). However, the pregnancy rate was significantly decreased when the difference was greater than 24 h (0 to 35%; P << 0.05). Re-cloning can be used for many purposes, and synchronization between donors and recipients may be an important factor for further success of nuclear transfer." @default.
- W2017649369 created "2016-06-24" @default.
- W2017649369 creator A5009477046 @default.
- W2017649369 creator A5015560461 @default.
- W2017649369 creator A5018623158 @default.
- W2017649369 creator A5040849139 @default.
- W2017649369 creator A5050743222 @default.
- W2017649369 creator A5057718398 @default.
- W2017649369 creator A5070620213 @default.
- W2017649369 creator A5072053019 @default.
- W2017649369 creator A5081557026 @default.
- W2017649369 date "2007-01-01" @default.
- W2017649369 modified "2023-09-23" @default.
- W2017649369 title "73 RE-CLONING BY SOMATIC CELL NUCLEAR TRANSFER FROM A CLONED KOREAN NATIVE GOAT" @default.
- W2017649369 doi "https://doi.org/10.1071/rdv19n1ab73" @default.
- W2017649369 hasPublicationYear "2007" @default.
- W2017649369 type Work @default.
- W2017649369 sameAs 2017649369 @default.
- W2017649369 citedByCount "0" @default.
- W2017649369 crossrefType "journal-article" @default.
- W2017649369 hasAuthorship W2017649369A5009477046 @default.
- W2017649369 hasAuthorship W2017649369A5015560461 @default.
- W2017649369 hasAuthorship W2017649369A5018623158 @default.
- W2017649369 hasAuthorship W2017649369A5040849139 @default.
- W2017649369 hasAuthorship W2017649369A5050743222 @default.
- W2017649369 hasAuthorship W2017649369A5057718398 @default.
- W2017649369 hasAuthorship W2017649369A5070620213 @default.
- W2017649369 hasAuthorship W2017649369A5072053019 @default.
- W2017649369 hasAuthorship W2017649369A5081557026 @default.
- W2017649369 hasConcept C104317684 @default.
- W2017649369 hasConcept C121050878 @default.
- W2017649369 hasConcept C134305767 @default.
- W2017649369 hasConcept C14814345 @default.
- W2017649369 hasConcept C153911025 @default.
- W2017649369 hasConcept C160099875 @default.
- W2017649369 hasConcept C163949779 @default.
- W2017649369 hasConcept C16685009 @default.
- W2017649369 hasConcept C185592680 @default.
- W2017649369 hasConcept C196843134 @default.
- W2017649369 hasConcept C199360897 @default.
- W2017649369 hasConcept C202438428 @default.
- W2017649369 hasConcept C2776690073 @default.
- W2017649369 hasConcept C2778177303 @default.
- W2017649369 hasConcept C41008148 @default.
- W2017649369 hasConcept C54355233 @default.
- W2017649369 hasConcept C71924100 @default.
- W2017649369 hasConcept C86803240 @default.
- W2017649369 hasConcept C87073359 @default.
- W2017649369 hasConcept C95444343 @default.
- W2017649369 hasConceptScore W2017649369C104317684 @default.
- W2017649369 hasConceptScore W2017649369C121050878 @default.
- W2017649369 hasConceptScore W2017649369C134305767 @default.
- W2017649369 hasConceptScore W2017649369C14814345 @default.
- W2017649369 hasConceptScore W2017649369C153911025 @default.
- W2017649369 hasConceptScore W2017649369C160099875 @default.
- W2017649369 hasConceptScore W2017649369C163949779 @default.
- W2017649369 hasConceptScore W2017649369C16685009 @default.
- W2017649369 hasConceptScore W2017649369C185592680 @default.
- W2017649369 hasConceptScore W2017649369C196843134 @default.
- W2017649369 hasConceptScore W2017649369C199360897 @default.
- W2017649369 hasConceptScore W2017649369C202438428 @default.
- W2017649369 hasConceptScore W2017649369C2776690073 @default.
- W2017649369 hasConceptScore W2017649369C2778177303 @default.
- W2017649369 hasConceptScore W2017649369C41008148 @default.
- W2017649369 hasConceptScore W2017649369C54355233 @default.
- W2017649369 hasConceptScore W2017649369C71924100 @default.
- W2017649369 hasConceptScore W2017649369C86803240 @default.
- W2017649369 hasConceptScore W2017649369C87073359 @default.
- W2017649369 hasConceptScore W2017649369C95444343 @default.
- W2017649369 hasIssue "1" @default.
- W2017649369 hasLocation W20176493691 @default.
- W2017649369 hasOpenAccess W2017649369 @default.
- W2017649369 hasPrimaryLocation W20176493691 @default.
- W2017649369 hasRelatedWork W1992361296 @default.
- W2017649369 hasRelatedWork W1993764803 @default.
- W2017649369 hasRelatedWork W2020247122 @default.
- W2017649369 hasRelatedWork W2033767072 @default.
- W2017649369 hasRelatedWork W2083451965 @default.
- W2017649369 hasRelatedWork W2167894697 @default.
- W2017649369 hasRelatedWork W2378094771 @default.
- W2017649369 hasRelatedWork W2391551638 @default.
- W2017649369 hasRelatedWork W2526517133 @default.
- W2017649369 hasRelatedWork W2551277998 @default.
- W2017649369 hasVolume "19" @default.
- W2017649369 isParatext "false" @default.
- W2017649369 isRetracted "false" @default.
- W2017649369 magId "2017649369" @default.
- W2017649369 workType "article" @default.