Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017669308> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W2017669308 endingPage "IN4" @default.
- W2017669308 startingPage "319" @default.
- W2017669308 abstract "α-Crystallin was isolated from total water-soluble lens extract by preparative zone electrophoresis on starch or Pevikon C870 blocks and purified by density gradient centrifugation and Sephadex chromatography. These preparations were treated with urea or sodium dodecyl sulphate and submitted to electrophoresis on polyacrylamide gels containing either urea or sodium dodecyl sulphate. Whereas in 7 M urea a large number of zones was detected, only three bands were observed in 1% sodium dodecyl sulphate. On the other hand, the sedimentation coefficient had the same value in both media. We re-investigated the N-terminal amino acid content in the starch block preparations and compared the result with that obtained from the preparations isolated according to our new procedure. The concentration of dinitrophenyl-glutamic acid was lower in the purified samples. Urea-treated samples had the same concentration of N-terminal glutamic acid whereas the concentration of the “trace” end-groups did not change. Sedimentation-diffusion equilibrium in the analytical ultracentrifuge revealed a slight heterogeneity in the purified samples. Electron micrographs of electrophoretic and of further purified samples were very similar. At neutral or slightly alkaline pH they showed almost uniform spherical aggregates in which a substructure was observed. At acid pH, coiled filaments rather than small globules could be demonstrated." @default.
- W2017669308 created "2016-06-24" @default.
- W2017669308 creator A5003561020 @default.
- W2017669308 creator A5035292294 @default.
- W2017669308 creator A5067559427 @default.
- W2017669308 creator A5072503686 @default.
- W2017669308 date "1965-12-01" @default.
- W2017669308 modified "2023-10-18" @default.
- W2017669308 title "On the subunits of α-crystallin" @default.
- W2017669308 cites W1995378328 @default.
- W2017669308 cites W1997884853 @default.
- W2017669308 cites W1999634987 @default.
- W2017669308 cites W2008046914 @default.
- W2017669308 cites W2029423652 @default.
- W2017669308 cites W2035662770 @default.
- W2017669308 cites W2086579570 @default.
- W2017669308 cites W2102687062 @default.
- W2017669308 cites W2423402891 @default.
- W2017669308 cites W2469682408 @default.
- W2017669308 doi "https://doi.org/10.1016/s0014-4835(65)80047-1" @default.
- W2017669308 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/5867353" @default.
- W2017669308 hasPublicationYear "1965" @default.
- W2017669308 type Work @default.
- W2017669308 sameAs 2017669308 @default.
- W2017669308 citedByCount "41" @default.
- W2017669308 crossrefType "journal-article" @default.
- W2017669308 hasAuthorship W2017669308A5003561020 @default.
- W2017669308 hasAuthorship W2017669308A5035292294 @default.
- W2017669308 hasAuthorship W2017669308A5067559427 @default.
- W2017669308 hasAuthorship W2017669308A5072503686 @default.
- W2017669308 hasConcept C1388644 @default.
- W2017669308 hasConcept C163994747 @default.
- W2017669308 hasConcept C178790620 @default.
- W2017669308 hasConcept C181199279 @default.
- W2017669308 hasConcept C185592680 @default.
- W2017669308 hasConcept C2778913249 @default.
- W2017669308 hasConcept C2780365088 @default.
- W2017669308 hasConcept C40250595 @default.
- W2017669308 hasConcept C43617362 @default.
- W2017669308 hasConcept C46749446 @default.
- W2017669308 hasConcept C537181965 @default.
- W2017669308 hasConcept C54689828 @default.
- W2017669308 hasConcept C55493867 @default.
- W2017669308 hasConcept C7623868 @default.
- W2017669308 hasConceptScore W2017669308C1388644 @default.
- W2017669308 hasConceptScore W2017669308C163994747 @default.
- W2017669308 hasConceptScore W2017669308C178790620 @default.
- W2017669308 hasConceptScore W2017669308C181199279 @default.
- W2017669308 hasConceptScore W2017669308C185592680 @default.
- W2017669308 hasConceptScore W2017669308C2778913249 @default.
- W2017669308 hasConceptScore W2017669308C2780365088 @default.
- W2017669308 hasConceptScore W2017669308C40250595 @default.
- W2017669308 hasConceptScore W2017669308C43617362 @default.
- W2017669308 hasConceptScore W2017669308C46749446 @default.
- W2017669308 hasConceptScore W2017669308C537181965 @default.
- W2017669308 hasConceptScore W2017669308C54689828 @default.
- W2017669308 hasConceptScore W2017669308C55493867 @default.
- W2017669308 hasConceptScore W2017669308C7623868 @default.
- W2017669308 hasIssue "4" @default.
- W2017669308 hasLocation W20176693081 @default.
- W2017669308 hasLocation W20176693082 @default.
- W2017669308 hasOpenAccess W2017669308 @default.
- W2017669308 hasPrimaryLocation W20176693081 @default.
- W2017669308 hasRelatedWork W1451221016 @default.
- W2017669308 hasRelatedWork W1971077832 @default.
- W2017669308 hasRelatedWork W1973880664 @default.
- W2017669308 hasRelatedWork W1981805561 @default.
- W2017669308 hasRelatedWork W2017395222 @default.
- W2017669308 hasRelatedWork W2028016149 @default.
- W2017669308 hasRelatedWork W2415532047 @default.
- W2017669308 hasRelatedWork W2994340503 @default.
- W2017669308 hasRelatedWork W332596026 @default.
- W2017669308 hasRelatedWork W5966800 @default.
- W2017669308 hasVolume "4" @default.
- W2017669308 isParatext "false" @default.
- W2017669308 isRetracted "false" @default.
- W2017669308 magId "2017669308" @default.
- W2017669308 workType "article" @default.