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- W2017669945 abstract "An energy dispersive X-ray fluorescence analysis was applied for determining the spatial (two-dimensional) distribution of elemental concentrations in rat brain sections. Freeze-dried brain sections prepared from normal and ischemic rats with middle cerebral artery occlusion were scanned with a collimated X-ray beam (0.18 mm in diameter, 50-kV acceleration voltage). The fluorescent Kα X-rays of P, S, Cl, and K were detectable, so that the two-dimensional distribution of fluorescent X-ray intensities could be determined for these elements. Furthermore, quantitative determination was possible for P and K by using the fundamental parameter technique. However, the accurate determination of Na and Ca was difficult, because of the low energy of Kα X-ray of Na, and the interference of K-Kβ with Ca-Kα. The change in elemental concentrations in ischemic tissue, including the decrease in K concentration and increase in Cl concentration, was demonstrated by this method as a two-dimensional contour map. Since it is possible to obtain a pictorial representation of the elemental concentration in tissue sections, this method may be useful to evaluate the ionic changes in injured brain tissue in relation to histological or autoradiographical observations." @default.
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- W2017669945 date "2000-07-01" @default.
- W2017669945 modified "2023-10-16" @default.
- W2017669945 title "Determination of the spatial distribution of major elements in the rat brain with X-ray fluorescence analysis" @default.
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- W2017669945 doi "https://doi.org/10.1016/s0165-0270(00)00231-4" @default.
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