FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017672977> ?p ?o ?g. }

• W2017672977 endingPage "6229" @default.
• W2017672977 startingPage "6224" @default.
• W2017672977 abstract "The magnitude and duration of a cytosolic Ca(2+) release can potentially be altered by changing the rate of Ca(2+) efflux. In plant cells, Ca(2+) efflux from the cytoplasm is mediated by H(+)/Ca(2+)-antiporters and two types of Ca(2+)-ATPases. ACA2 was recently identified as a calmodulin-regulated Ca(2+)-pump located in the endoplasmic reticulum. Here, we show that phosphorylation of its N-terminal regulatory domain by a Ca(2+)-dependent protein kinase (CDPK isoform CPK1), inhibits both basal activity ( approximately 10%) and calmodulin stimulation ( approximately 75%), as shown by Ca(2+)-transport assays with recombinant enzyme expressed in yeast. A CDPK phosphorylation site was mapped to Ser(45) near a calmodulin binding site, using a fusion protein containing the N-terminal domain as an in vitro substrate for a recombinant CPK1. In a full-length enzyme, an Ala substitution for Ser(45) (S45/A) completely blocked the observed CDPK inhibition of both basal and calmodulin-stimulated activities. An Asp substitution (S45/D) mimicked phosphoinhibition, indicating that a negative charge at this position is sufficient to account for phosphoinhibition. Interestingly, prior binding of calmodulin blocked phosphorylation. This suggests that, once ACA2 binds calmodulin, its activation state becomes resistant to phosphoinhibition. These results support the hypothesis that ACA2 activity is regulated as the balance between the initial kinetics of calmodulin stimulation and CDPK inhibition, providing an example in plants for a potential point of crosstalk between two different Ca(2+)-signaling pathways." @default.
• W2017672977 created "2016-06-24" @default.
• W2017672977 creator A5010154886 @default.
• W2017672977 creator A5015528389 @default.
• W2017672977 creator A5059253020 @default.
• W2017672977 date "2000-05-23" @default.
• W2017672977 modified "2023-10-16" @default.
• W2017672977 title "A calcium-dependent protein kinase can inhibit a calmodulin-stimulated Ca ²⁺ pump (ACA2) located in the endoplasmic reticulum of <i>Arabidopsis</i>" @default.
• W2017672977 cites W1002836587 @default.
• W2017672977 cites W144778061 @default.
• W2017672977 cites W1537228249 @default.
• W2017672977 cites W1593180325 @default.
• W2017672977 cites W1973459905 @default.
• W2017672977 cites W2000347653 @default.
• W2017672977 cites W2002027111 @default.
• W2017672977 cites W2010161333 @default.
• W2017672977 cites W2013307216 @default.
• W2017672977 cites W2017599638 @default.
• W2017672977 cites W2033738324 @default.
• W2017672977 cites W2034273977 @default.
• W2017672977 cites W2034526004 @default.
• W2017672977 cites W2042786900 @default.
• W2017672977 cites W2049534556 @default.
• W2017672977 cites W2051493425 @default.
• W2017672977 cites W2053108652 @default.
• W2017672977 cites W2060448496 @default.
• W2017672977 cites W2060759606 @default.
• W2017672977 cites W2064024936 @default.
• W2017672977 cites W2067999317 @default.
• W2017672977 cites W2072682674 @default.
• W2017672977 cites W2078041570 @default.
• W2017672977 cites W2091005592 @default.
• W2017672977 cites W2104120677 @default.
• W2017672977 cites W2107124162 @default.
• W2017672977 cites W2108367015 @default.
• W2017672977 cites W2118342939 @default.
• W2017672977 cites W2120649594 @default.
• W2017672977 cites W2122086142 @default.
• W2017672977 cites W2125767746 @default.
• W2017672977 cites W2127780414 @default.
• W2017672977 cites W2128017307 @default.
• W2017672977 cites W2129673771 @default.
• W2017672977 cites W2135080621 @default.
• W2017672977 cites W2138964735 @default.
• W2017672977 cites W2156650130 @default.
• W2017672977 cites W2164331942 @default.
• W2017672977 cites W2164955616 @default.
• W2017672977 cites W4253656315 @default.
• W2017672977 doi "https://doi.org/10.1073/pnas.97.11.6224" @default.
• W2017672977 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/18586" @default.
• W2017672977 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10823962" @default.
• W2017672977 hasPublicationYear "2000" @default.
• W2017672977 type Work @default.
• W2017672977 sameAs 2017672977 @default.
• W2017672977 citedByCount "167" @default.
• W2017672977 countsByYear W20176729772012 @default.
• W2017672977 countsByYear W20176729772013 @default.
• W2017672977 countsByYear W20176729772014 @default.
• W2017672977 countsByYear W20176729772015 @default.
• W2017672977 countsByYear W20176729772016 @default.
• W2017672977 countsByYear W20176729772017 @default.
• W2017672977 countsByYear W20176729772018 @default.
• W2017672977 countsByYear W20176729772019 @default.
• W2017672977 countsByYear W20176729772020 @default.
• W2017672977 countsByYear W20176729772021 @default.
• W2017672977 countsByYear W20176729772022 @default.
• W2017672977 countsByYear W20176729772023 @default.
• W2017672977 crossrefType "journal-article" @default.
• W2017672977 hasAuthorship W2017672977A5010154886 @default.
• W2017672977 hasAuthorship W2017672977A5015528389 @default.
• W2017672977 hasAuthorship W2017672977A5059253020 @default.
• W2017672977 hasBestOaLocation W20176729771 @default.
• W2017672977 hasConcept C11960822 @default.
• W2017672977 hasConcept C153911025 @default.
• W2017672977 hasConcept C158617107 @default.
• W2017672977 hasConcept C181199279 @default.
• W2017672977 hasConcept C185592680 @default.
• W2017672977 hasConcept C23265538 @default.
• W2017672977 hasConcept C29688787 @default.
• W2017672977 hasConcept C55493867 @default.
• W2017672977 hasConcept C86803240 @default.
• W2017672977 hasConcept C935647 @default.
• W2017672977 hasConcept C95444343 @default.
• W2017672977 hasConcept C97029542 @default.
• W2017672977 hasConceptScore W2017672977C11960822 @default.
• W2017672977 hasConceptScore W2017672977C153911025 @default.
• W2017672977 hasConceptScore W2017672977C158617107 @default.
• W2017672977 hasConceptScore W2017672977C181199279 @default.
• W2017672977 hasConceptScore W2017672977C185592680 @default.
• W2017672977 hasConceptScore W2017672977C23265538 @default.
• W2017672977 hasConceptScore W2017672977C29688787 @default.
• W2017672977 hasConceptScore W2017672977C55493867 @default.
• W2017672977 hasConceptScore W2017672977C86803240 @default.
• W2017672977 hasConceptScore W2017672977C935647 @default.
• W2017672977 hasConceptScore W2017672977C95444343 @default.
• W2017672977 hasConceptScore W2017672977C97029542 @default.
• W2017672977 hasIssue "11" @default.
• W2017672977 hasLocation W20176729771 @default.

• next