FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017680056> ?p ?o ?g. }

• W2017680056 endingPage "10534" @default.
• W2017680056 startingPage "10526" @default.
• W2017680056 abstract "Cystathionine β-synthase (CBS) is a modular enzyme which catalyzes condensation of serine with homocysteine. Cross-talk between the catalytic core and the C-terminal regulatory domain modulates the enzyme activity. The regulatory domain imposes an autoinhibition action that is alleviated by S-adenosyl-l-methionine (AdoMet) binding, by deletion of the C-terminal regulatory module, or by thermal activation. The atomic mechanisms of the CBS allostery have not yet been sufficiently explained. Using pulse proteolysis in urea gradient and proteolytic kinetics with thermolysin under native conditions, we demonstrated that autoinhibition is associated with changes in conformational stability and with sterical hindrance of the catalytic core. To determine the contact area between the catalytic core and the autoinhibitory module of the CBS protein, we compared side-chain reactivity of the truncated CBS lacking the regulatory domain (45CBS) and of the full-length enzyme (wtCBS) using covalent labeling by six different modification agents and subsequent mass spectrometry. Fifty modification sites were identified in 45CBS, and four of them were not labeled in wtCBS. One differentially reactive site (cluster W408/W409/W410) is a part of the linker between the domains. The other three residues (K172 and/or K177, R336, and K384) are located in the same region of the 45CBS crystal structure; computational modeling showed that these amino acid side chains potentially form a regulatory interface in CBS protein. Subtle differences at CBS surface indicate that enzyme activity is not regulated by conformational conversions but more likely by different allosteric mechanisms." @default.
• W2017680056 created "2016-06-24" @default.
• W2017680056 creator A5053282952 @default.
• W2017680056 creator A5055797941 @default.
• W2017680056 creator A5062105757 @default.
• W2017680056 creator A5068377776 @default.
• W2017680056 creator A5071980210 @default.
• W2017680056 creator A5089803719 @default.
• W2017680056 date "2010-11-17" @default.
• W2017680056 modified "2023-09-26" @default.
• W2017680056 title "Cross-Talk between the Catalytic Core and the Regulatory Domain in Cystathionine β-Synthase: Study by Differential Covalent Labeling and Computational Modeling" @default.
• W2017680056 cites W1586592493 @default.
• W2017680056 cites W1964410776 @default.
• W2017680056 cites W1965403325 @default.
• W2017680056 cites W1967535331 @default.
• W2017680056 cites W1968255611 @default.
• W2017680056 cites W1969116247 @default.
• W2017680056 cites W1974686502 @default.
• W2017680056 cites W1975651140 @default.
• W2017680056 cites W1976674660 @default.
• W2017680056 cites W1983995089 @default.
• W2017680056 cites W1988357957 @default.
• W2017680056 cites W1990324969 @default.
• W2017680056 cites W1991484280 @default.
• W2017680056 cites W1992735259 @default.
• W2017680056 cites W1994485464 @default.
• W2017680056 cites W2005474992 @default.
• W2017680056 cites W2006481826 @default.
• W2017680056 cites W2007549861 @default.
• W2017680056 cites W2011221486 @default.
• W2017680056 cites W2014763486 @default.
• W2017680056 cites W2021054184 @default.
• W2017680056 cites W2023454012 @default.
• W2017680056 cites W2039474401 @default.
• W2017680056 cites W2046034113 @default.
• W2017680056 cites W2047296248 @default.
• W2017680056 cites W2049598343 @default.
• W2017680056 cites W2060259273 @default.
• W2017680056 cites W2062665530 @default.
• W2017680056 cites W2065283382 @default.
• W2017680056 cites W2071790569 @default.
• W2017680056 cites W2072349322 @default.
• W2017680056 cites W2074187513 @default.
• W2017680056 cites W2074913671 @default.
• W2017680056 cites W2076377500 @default.
• W2017680056 cites W2080106524 @default.
• W2017680056 cites W2085457861 @default.
• W2017680056 cites W2087927611 @default.
• W2017680056 cites W2088659033 @default.
• W2017680056 cites W2089859405 @default.
• W2017680056 cites W2094612469 @default.
• W2017680056 cites W2095420958 @default.
• W2017680056 cites W2103647536 @default.
• W2017680056 cites W2104199818 @default.
• W2017680056 cites W2106154817 @default.
• W2017680056 cites W2111729275 @default.
• W2017680056 cites W2112822852 @default.
• W2017680056 cites W2133258578 @default.
• W2017680056 cites W2133712572 @default.
• W2017680056 cites W2139680667 @default.
• W2017680056 cites W2151457629 @default.
• W2017680056 cites W2158714788 @default.
• W2017680056 cites W2159322886 @default.
• W2017680056 cites W2159998622 @default.
• W2017680056 cites W4210623056 @default.
• W2017680056 doi "https://doi.org/10.1021/bi101384m" @default.
• W2017680056 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3146298" @default.
• W2017680056 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21062078" @default.
• W2017680056 hasPublicationYear "2010" @default.
• W2017680056 type Work @default.
• W2017680056 sameAs 2017680056 @default.
• W2017680056 citedByCount "17" @default.
• W2017680056 countsByYear W20176800562012 @default.
• W2017680056 countsByYear W20176800562013 @default.
• W2017680056 countsByYear W20176800562014 @default.
• W2017680056 countsByYear W20176800562015 @default.
• W2017680056 countsByYear W20176800562016 @default.
• W2017680056 countsByYear W20176800562019 @default.
• W2017680056 countsByYear W20176800562020 @default.
• W2017680056 crossrefType "journal-article" @default.
• W2017680056 hasAuthorship W2017680056A5053282952 @default.
• W2017680056 hasAuthorship W2017680056A5055797941 @default.
• W2017680056 hasAuthorship W2017680056A5062105757 @default.
• W2017680056 hasAuthorship W2017680056A5068377776 @default.
• W2017680056 hasAuthorship W2017680056A5071980210 @default.
• W2017680056 hasAuthorship W2017680056A5089803719 @default.
• W2017680056 hasBestOaLocation W20176800561 @default.
• W2017680056 hasConcept C12554922 @default.
• W2017680056 hasConcept C16525657 @default.
• W2017680056 hasConcept C166342909 @default.
• W2017680056 hasConcept C181199279 @default.
• W2017680056 hasConcept C185592680 @default.
• W2017680056 hasConcept C2779201268 @default.
• W2017680056 hasConcept C47701112 @default.
• W2017680056 hasConcept C55493867 @default.
• W2017680056 hasConcept C71240020 @default.
• W2017680056 hasConcept C86803240 @default.
• W2017680056 hasConceptScore W2017680056C12554922 @default.

• next