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- W2017682208 abstract "Three types of platelet concentrates (PC) are compared: PC either processed with the platelet-rich plasma (PRP) or the Buffy coat (BC) method from whole blood units and PC obtained by apheresis. Leuko-reduction (LR) pre-storage is advocated to improve quality of the PC during storage and reduce adverse reactions in recipients. Standardization of methods allow preparation of PC with comparable yields of ∼400 × 109 platelets in pooled non-LR-PRP, ∼370 × 109 in pooled LR–BC–PC and in LR apheresis PC the number of platelets can be targeted on 350 × 109 or more with devices of various manufacturers. While viral transmission can be prevented by outstanding laboratory tests, the risk of bacterial contamination should be reduced by improved arm disinfection, deviation of the first 20–30 ml of blood and culture or rapid detection assays of the PC pre-issue. In a large prospective multicenter trial no significant difference was observed between cultures of apheresis PC (n = 15,198): 0.09% confirmed positive units versus 0.06% in pooled BC–PC (n = 37,045), respectively. Though platelet activation as measured by CD62 expression may differ in vitro in PC obtained with various apheresis equipment, and also between PC processed with the two whole blood methods there is scarce literature about the clinical impact of these findings. In conclusion the final products of LR–PC derived from whole blood or obtained by apheresis can be comparable, provided the critical steps of the processing method are identified and covered and the process is in control." @default.
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- W2017682208 date "2009-10-01" @default.
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- W2017682208 title "Pooled platelet concentrates: An alternative to single donor apheresis platelets?" @default.
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- W2017682208 doi "https://doi.org/10.1016/j.transci.2009.07.003" @default.
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