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- W2017687764 abstract "The present study was undertaken to investigate growth kinetics and time-dependent change in global expression of Escherichia coli O157∶H7 Sakai upon an abrupt downshift in water activity (aw). Based on viable count data, shifting E. coli from aw 0.993 to aw 0.985 or less caused an apparent loss, then recovery, of culturability. Exponential growth then resumed at a rate characteristic for the aw imposed. To understand the responses of this pathogen to abrupt osmotic stress, we employed an integrated genomic and proteomic approach to characterize its cellular response during exposure to a rapid downshift but still within the growth range from aw 0.993 to aw 0.967. Of particular interest, genes and proteins with cell envelope-related functions were induced during the initial loss and subsequent recovery of culturability. This implies that cells undergo remodeling of their envelope composition, enabling them to adapt to osmotic stress. Growth at low aw, however, involved up-regulating additional genes and proteins, which are involved in the biosynthesis of specific amino acids, and carbohydrate catabolism and energy generation. This suggests their important role in facilitating growth under such stress. Finally, we highlighted the ability of E. coli to activate multiple stress responses by transiently inducing the RpoE and RpoH regulons to control protein misfolding, while simultaneously activating the master stress regulator RpoS to mediate long-term adaptation to hyperosmolality. This investigation extends our understanding of the potential mechanisms used by pathogenic E. coli to adapt, survive and grow under osmotic stress, which could potentially be exploited to aid the selection and/or development of novel strategies to inactivate this pathogen." @default.
- W2017687764 created "2016-06-24" @default.
- W2017687764 creator A5025253715 @default.
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- W2017687764 creator A5032576358 @default.
- W2017687764 creator A5035706538 @default.
- W2017687764 creator A5079858280 @default.
- W2017687764 date "2014-03-03" @default.
- W2017687764 modified "2023-10-17" @default.
- W2017687764 title "Global Genome Response of Escherichia coli O157∶H7 Sakai during Dynamic Changes in Growth Kinetics Induced by an Abrupt Downshift in Water Activity" @default.
- W2017687764 cites W1436747525 @default.
- W2017687764 cites W1501009591 @default.
- W2017687764 cites W1516076610 @default.
- W2017687764 cites W1531339633 @default.
- W2017687764 cites W1550429296 @default.
- W2017687764 cites W1559715495 @default.
- W2017687764 cites W1668246716 @default.
- W2017687764 cites W1863386058 @default.
- W2017687764 cites W1914117083 @default.
- W2017687764 cites W1964000156 @default.
- W2017687764 cites W1966462828 @default.
- W2017687764 cites W1966621931 @default.
- W2017687764 cites W1969757209 @default.
- W2017687764 cites W1980243031 @default.
- W2017687764 cites W1982088862 @default.
- W2017687764 cites W1984628925 @default.
- W2017687764 cites W1986821060 @default.
- W2017687764 cites W2000372799 @default.
- W2017687764 cites W2000424161 @default.
- W2017687764 cites W2001552323 @default.
- W2017687764 cites W2006061929 @default.
- W2017687764 cites W2007872530 @default.
- W2017687764 cites W2010264737 @default.
- W2017687764 cites W2015096214 @default.
- W2017687764 cites W2015556811 @default.
- W2017687764 cites W2023476707 @default.
- W2017687764 cites W2032470538 @default.
- W2017687764 cites W2034455997 @default.
- W2017687764 cites W2038613897 @default.
- W2017687764 cites W2039160511 @default.
- W2017687764 cites W2041025550 @default.
- W2017687764 cites W2041671414 @default.
- W2017687764 cites W2046232966 @default.
- W2017687764 cites W2047274781 @default.
- W2017687764 cites W2049848001 @default.
- W2017687764 cites W2051048419 @default.
- W2017687764 cites W2051496427 @default.
- W2017687764 cites W2053894359 @default.
- W2017687764 cites W2053944527 @default.
- W2017687764 cites W2062440160 @default.
- W2017687764 cites W2063547978 @default.
- W2017687764 cites W2065951509 @default.
- W2017687764 cites W2067649592 @default.
- W2017687764 cites W2069281061 @default.
- W2017687764 cites W2069724548 @default.
- W2017687764 cites W2074336912 @default.
- W2017687764 cites W2084952975 @default.
- W2017687764 cites W2093262908 @default.
- W2017687764 cites W2094148851 @default.
- W2017687764 cites W2094953050 @default.
- W2017687764 cites W2099836442 @default.
- W2017687764 cites W2103016908 @default.
- W2017687764 cites W2104833884 @default.
- W2017687764 cites W2105028012 @default.
- W2017687764 cites W2105788036 @default.
- W2017687764 cites W2106490639 @default.
- W2017687764 cites W2109947095 @default.
- W2017687764 cites W2111240057 @default.
- W2017687764 cites W2112078820 @default.
- W2017687764 cites W2112369097 @default.
- W2017687764 cites W2115606218 @default.
- W2017687764 cites W2116307528 @default.
- W2017687764 cites W2122560605 @default.
- W2017687764 cites W2125323026 @default.
- W2017687764 cites W2130706354 @default.
- W2017687764 cites W2131090645 @default.
- W2017687764 cites W2133342418 @default.
- W2017687764 cites W2134701031 @default.
- W2017687764 cites W2137178857 @default.
- W2017687764 cites W2139782481 @default.
- W2017687764 cites W2140516217 @default.
- W2017687764 cites W2141590350 @default.
- W2017687764 cites W2153562609 @default.
- W2017687764 cites W2155031737 @default.
- W2017687764 cites W2157918066 @default.
- W2017687764 cites W2158279787 @default.
- W2017687764 cites W2159686118 @default.
- W2017687764 cites W2163109978 @default.
- W2017687764 cites W2166157766 @default.
- W2017687764 cites W2166489555 @default.
- W2017687764 cites W2167044013 @default.
- W2017687764 cites W2170333347 @default.
- W2017687764 cites W2170949754 @default.
- W2017687764 cites W2170989872 @default.
- W2017687764 cites W2171671996 @default.
- W2017687764 cites W2486554223 @default.
- W2017687764 cites W4243353674 @default.
- W2017687764 cites W7642488 @default.