FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017694016> ?p ?o ?g. }

• W2017694016 endingPage "154" @default.
• W2017694016 startingPage "149" @default.
• W2017694016 abstract "Spiroplasma citri is a phloem-limited bacterium causing citrus stubborn disease (CSD). Isolation and culturing of S. citri is technically demanding and time consuming. S. citri is typically low in titer and unevenly distributed in citrus, making reliable detection challenging. The current preferred detection method is polymerase chain reaction (PCR) assays with primers developed from sequences of S. citri housekeeping genes. Recent genome sequencing of S. citri revealed that the bacterium harbors multiple copies of prophage genes. Therefore, targeting multicopy prophage genes was hypothesized to improve sensitivity of PCR detection. Two primer sets, Php-orf1 and Php-orf3, were developed from conserved prophage sequences in the S. citri genome. These primer sets were used to evaluate detection sensitivity in SYBR Green-based quantitative PCR (qPCR) assays with 18 S. citri in cultures isolated from different hosts and locations. Prophage primer set Php-orf1 increased detection sensitivity by 4.91 and 3.65 cycle threshold (Cq) units compared with housekeeping gene primers for spiralin and P58 putative adhesin gene, respectively. Detection was slightly less sensitive for the Php-orf3 primer set at 3.02 and 1.76 Cq units, respectively, over the same housekeeping gene primers. The prophage primer sets were validated for qPCR detection with field samples from three citrus orchards in California's San Joaquin Valley collected from 2007 to 2013. The data showed that S. citri prophage sequences improved sensitivity for qPCR detection of S. citri-infected trees at least 10-fold and reduced the number of false-negative results. No false-positive samples were detected with any of the primer sets. The enhanced sensitivity resulted from the higher copy number of prophage genes in the S. citri genome and, thus, improved CSD diagnosis from field samples." @default.
• W2017694016 created "2016-06-24" @default.
• W2017694016 creator A5050577909 @default.
• W2017694016 creator A5053491216 @default.
• W2017694016 creator A5073641479 @default.
• W2017694016 creator A5084138890 @default.
• W2017694016 date "2015-01-01" @default.
• W2017694016 modified "2023-10-15" @default.
• W2017694016 title "Improved Real-Time PCR Diagnosis of Citrus Stubborn Disease by Targeting Prophage Genes of <i>Spiroplasma citri</i>" @default.
• W2017694016 cites W1964201368 @default.
• W2017694016 cites W1985989889 @default.
• W2017694016 cites W2003596199 @default.
• W2017694016 cites W2003617438 @default.
• W2017694016 cites W2003711589 @default.
• W2017694016 cites W2004617824 @default.
• W2017694016 cites W2005230001 @default.
• W2017694016 cites W2009975313 @default.
• W2017694016 cites W2011818090 @default.
• W2017694016 cites W2012303132 @default.
• W2017694016 cites W2019392109 @default.
• W2017694016 cites W2023977002 @default.
• W2017694016 cites W2028139360 @default.
• W2017694016 cites W2057953946 @default.
• W2017694016 cites W2059557960 @default.
• W2017694016 cites W2063617144 @default.
• W2017694016 cites W2069128651 @default.
• W2017694016 cites W2076673266 @default.
• W2017694016 cites W2092069947 @default.
• W2017694016 cites W2104214690 @default.
• W2017694016 cites W2105205851 @default.
• W2017694016 cites W2139760555 @default.
• W2017694016 cites W2142678478 @default.
• W2017694016 cites W2147330879 @default.
• W2017694016 cites W2159319664 @default.
• W2017694016 cites W2491510849 @default.
• W2017694016 cites W2501489917 @default.
• W2017694016 cites W2780552167 @default.
• W2017694016 cites W84311565 @default.
• W2017694016 doi "https://doi.org/10.1094/pdis-06-14-0572-re" @default.
• W2017694016 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30699732" @default.
• W2017694016 hasPublicationYear "2015" @default.
• W2017694016 type Work @default.
• W2017694016 sameAs 2017694016 @default.
• W2017694016 citedByCount "17" @default.
• W2017694016 countsByYear W20176940162016 @default.
• W2017694016 countsByYear W20176940162017 @default.
• W2017694016 countsByYear W20176940162019 @default.
• W2017694016 countsByYear W20176940162020 @default.
• W2017694016 countsByYear W20176940162021 @default.
• W2017694016 countsByYear W20176940162022 @default.
• W2017694016 countsByYear W20176940162023 @default.
• W2017694016 crossrefType "journal-article" @default.
• W2017694016 hasAuthorship W2017694016A5050577909 @default.
• W2017694016 hasAuthorship W2017694016A5053491216 @default.
• W2017694016 hasAuthorship W2017694016A5073641479 @default.
• W2017694016 hasAuthorship W2017694016A5084138890 @default.
• W2017694016 hasBestOaLocation W20176940161 @default.
• W2017694016 hasConcept C104317684 @default.
• W2017694016 hasConcept C141231307 @default.
• W2017694016 hasConcept C150194340 @default.
• W2017694016 hasConcept C159047783 @default.
• W2017694016 hasConcept C178790620 @default.
• W2017694016 hasConcept C185592680 @default.
• W2017694016 hasConcept C2776441376 @default.
• W2017694016 hasConcept C2777563447 @default.
• W2017694016 hasConcept C33922130 @default.
• W2017694016 hasConcept C49105822 @default.
• W2017694016 hasConcept C54355233 @default.
• W2017694016 hasConcept C547475151 @default.
• W2017694016 hasConcept C73445445 @default.
• W2017694016 hasConcept C86803240 @default.
• W2017694016 hasConcept C89423630 @default.
• W2017694016 hasConceptScore W2017694016C104317684 @default.
• W2017694016 hasConceptScore W2017694016C141231307 @default.
• W2017694016 hasConceptScore W2017694016C150194340 @default.
• W2017694016 hasConceptScore W2017694016C159047783 @default.
• W2017694016 hasConceptScore W2017694016C178790620 @default.
• W2017694016 hasConceptScore W2017694016C185592680 @default.
• W2017694016 hasConceptScore W2017694016C2776441376 @default.
• W2017694016 hasConceptScore W2017694016C2777563447 @default.
• W2017694016 hasConceptScore W2017694016C33922130 @default.
• W2017694016 hasConceptScore W2017694016C49105822 @default.
• W2017694016 hasConceptScore W2017694016C54355233 @default.
• W2017694016 hasConceptScore W2017694016C547475151 @default.
• W2017694016 hasConceptScore W2017694016C73445445 @default.
• W2017694016 hasConceptScore W2017694016C86803240 @default.
• W2017694016 hasConceptScore W2017694016C89423630 @default.
• W2017694016 hasIssue "1" @default.
• W2017694016 hasLocation W20176940161 @default.
• W2017694016 hasLocation W20176940162 @default.
• W2017694016 hasOpenAccess W2017694016 @default.
• W2017694016 hasPrimaryLocation W20176940161 @default.
• W2017694016 hasRelatedWork W1442603624 @default.
• W2017694016 hasRelatedWork W2017694016 @default.
• W2017694016 hasRelatedWork W2033995917 @default.
• W2017694016 hasRelatedWork W2035046591 @default.
• W2017694016 hasRelatedWork W2093909589 @default.
• W2017694016 hasRelatedWork W2124703740 @default.

• next