FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017694705> ?p ?o ?g. }

• W2017694705 endingPage "8556" @default.
• W2017694705 startingPage "8548" @default.
• W2017694705 abstract "Restenosis is the formation of blockages occurring at the site of angioplasty or stent placement. In order to avoid such blockages, the suppression of smooth muscle cells near the implanted stent is required. The Akt1 protein is known to be responsible for cellular proliferation, and specific inhibition of Akt1 gene expression results in the retardation of cell growth. To take advantage of these benefits, we developed a new delivery technique for Akt1 siRNA nanoparticles from a hyaluronic acid (HA)-coated stent surface. For this purpose, the disulfide cross-linked low molecular polyethyleneimine (PEI) (ssPEI) was used as a gene delivery carrier because disulfide bonds are stable in an oxidative extracellular environment but degrade rapidly in reductive intracellular environments. In this study, Akt1 siRNA showed efficient ionic interaction with the ssPEI carrier, which was confirmed by polyacrylamide gel electrophoresis. Akt1 siRNA/ssPEI nanoparticles (ASNs) were immobilized on the HA-coated stent surface and exhibited stable binding and localization, followed by time-dependent sustained release for intracellular uptake. Cellular viability on the nanoparticle-immobilized surface was assessed using A10 vascular smooth muscle cells, and the results revealed that immobilized ASNs exhibited negligible cytotoxicity against the adhering A10 cells. Transfection efficiency was quantified using a luciferase assay; the transgene expression of Akt1 suppression through the delivered Akt1 siRNA was measured using RT-PCR and western blot, demonstrating higher gene silencing efficiency when compared to other carriers. ASN coated on HA stents were deployed in the balloon-injured external iliac artery in rabbits in vivo. It was shown that the Akt1 released from the stent suppressed the growth of the smooth muscle at the peri-stent implantation area, resulting in the prevention of restenosis in the post-implantation phase." @default.
• W2017694705 created "2016-06-24" @default.
• W2017694705 creator A5000604839 @default.
• W2017694705 creator A5002541100 @default.
• W2017694705 creator A5003478304 @default.
• W2017694705 creator A5012040457 @default.
• W2017694705 creator A5017793519 @default.
• W2017694705 creator A5031117341 @default.
• W2017694705 creator A5049775286 @default.
• W2017694705 creator A5052540307 @default.
• W2017694705 creator A5053284042 @default.
• W2017694705 creator A5053728368 @default.
• W2017694705 creator A5066837534 @default.
• W2017694705 creator A5086377751 @default.
• W2017694705 date "2012-11-01" @default.
• W2017694705 modified "2023-10-03" @default.
• W2017694705 title "Suppression of post-angioplasty restenosis with an Akt1 siRNA-embedded coronary stent in a rabbit model" @default.
• W2017694705 cites W1582771675 @default.
• W2017694705 cites W1997145323 @default.
• W2017694705 cites W1997831486 @default.
• W2017694705 cites W2002069318 @default.
• W2017694705 cites W2006327162 @default.
• W2017694705 cites W2010655741 @default.
• W2017694705 cites W2016477175 @default.
• W2017694705 cites W2023941869 @default.
• W2017694705 cites W2028152693 @default.
• W2017694705 cites W2049576282 @default.
• W2017694705 cites W2051904824 @default.
• W2017694705 cites W2052643085 @default.
• W2017694705 cites W2057059824 @default.
• W2017694705 cites W2062949232 @default.
• W2017694705 cites W2063729029 @default.
• W2017694705 cites W2091140311 @default.
• W2017694705 cites W2092858957 @default.
• W2017694705 cites W2114567767 @default.
• W2017694705 cites W2143962152 @default.
• W2017694705 cites W2154156610 @default.
• W2017694705 cites W2182693089 @default.
• W2017694705 cites W2316154423 @default.
• W2017694705 doi "https://doi.org/10.1016/j.biomaterials.2012.07.045" @default.
• W2017694705 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22940215" @default.
• W2017694705 hasPublicationYear "2012" @default.
• W2017694705 type Work @default.
• W2017694705 sameAs 2017694705 @default.
• W2017694705 citedByCount "49" @default.
• W2017694705 countsByYear W20176947052013 @default.
• W2017694705 countsByYear W20176947052014 @default.
• W2017694705 countsByYear W20176947052015 @default.
• W2017694705 countsByYear W20176947052016 @default.
• W2017694705 countsByYear W20176947052017 @default.
• W2017694705 countsByYear W20176947052018 @default.
• W2017694705 countsByYear W20176947052019 @default.
• W2017694705 countsByYear W20176947052021 @default.
• W2017694705 countsByYear W20176947052022 @default.
• W2017694705 countsByYear W20176947052023 @default.
• W2017694705 crossrefType "journal-article" @default.
• W2017694705 hasAuthorship W2017694705A5000604839 @default.
• W2017694705 hasAuthorship W2017694705A5002541100 @default.
• W2017694705 hasAuthorship W2017694705A5003478304 @default.
• W2017694705 hasAuthorship W2017694705A5012040457 @default.
• W2017694705 hasAuthorship W2017694705A5017793519 @default.
• W2017694705 hasAuthorship W2017694705A5031117341 @default.
• W2017694705 hasAuthorship W2017694705A5049775286 @default.
• W2017694705 hasAuthorship W2017694705A5052540307 @default.
• W2017694705 hasAuthorship W2017694705A5053284042 @default.
• W2017694705 hasAuthorship W2017694705A5053728368 @default.
• W2017694705 hasAuthorship W2017694705A5066837534 @default.
• W2017694705 hasAuthorship W2017694705A5086377751 @default.
• W2017694705 hasConcept C104317684 @default.
• W2017694705 hasConcept C109316439 @default.
• W2017694705 hasConcept C119056186 @default.
• W2017694705 hasConcept C12554922 @default.
• W2017694705 hasConcept C141071460 @default.
• W2017694705 hasConcept C150903083 @default.
• W2017694705 hasConcept C153911025 @default.
• W2017694705 hasConcept C154137905 @default.
• W2017694705 hasConcept C185592680 @default.
• W2017694705 hasConcept C192562407 @default.
• W2017694705 hasConcept C202751555 @default.
• W2017694705 hasConcept C207001950 @default.
• W2017694705 hasConcept C2776415932 @default.
• W2017694705 hasConcept C2778283817 @default.
• W2017694705 hasConcept C2778583881 @default.
• W2017694705 hasConcept C28406088 @default.
• W2017694705 hasConcept C54009773 @default.
• W2017694705 hasConcept C55493867 @default.
• W2017694705 hasConcept C62478195 @default.
• W2017694705 hasConcept C71924100 @default.
• W2017694705 hasConcept C75217442 @default.
• W2017694705 hasConcept C79879829 @default.
• W2017694705 hasConcept C86803240 @default.
• W2017694705 hasConcept C95444343 @default.
• W2017694705 hasConceptScore W2017694705C104317684 @default.
• W2017694705 hasConceptScore W2017694705C109316439 @default.
• W2017694705 hasConceptScore W2017694705C119056186 @default.
• W2017694705 hasConceptScore W2017694705C12554922 @default.
• W2017694705 hasConceptScore W2017694705C141071460 @default.
• W2017694705 hasConceptScore W2017694705C150903083 @default.

• next