FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017698526> ?p ?o ?g. }

• W2017698526 endingPage "428" @default.
• W2017698526 startingPage "415" @default.
• W2017698526 abstract "A virulent isolate of Edwardsiella ictaluri (AL-93-75), the causative agent of enteric septicaemia of catfish (ESC), was used to derive a lipopolysaccharide-reduced N-lauroylsarcosine outer-membrane protein (OMP) fraction vaccine. The OMP fraction was analyzed using sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) and compared to whole-cell lysate, purified lipopolysaccharide (LPS) and a crude cell-wall fraction. The OMP fraction contained less than 2% (W/V) LPS. SDS–PAGE showed that whole cell lysates contained 27 proteins from 107 to 14.3 kDa, whereas OMP contained nine proteins from 97 to 14.3 kDa, LPS contained two proteins at 45 and 37 kDa bands and a smear of bands below 14.3 kDa, and cell wall fraction contained 21 proteins from 97 to 8 kDa. Channel catfish, Ictalurus punctatus, were vaccinated with 12.5 μg/100 μl OMP and immunogenicity was confirmed by subsequent Western blots. Blots showed that 97, 80, and 19 kDa proteins were immunogenic. Rapid enzyme-linked immunosorbent assays (ELISA) demonstrated that OMP produced a weak, but observable antibody response by 21 days post injection. OMP concentrations of 3.13, 6.25, 12.5, 25, and 50 μg/100 μl total protein were tested for protective immunity. Marginal protection by relative percent survival (RPS) was only seen for fish injected with 12.5 μg/100 μl with RPSs between 55–67.5%. A booster dose of 12.5 μg/100 μl OMP did not significantly enhance protection." @default.
• W2017698526 created "2016-06-24" @default.
• W2017698526 creator A5021887441 @default.
• W2017698526 creator A5024503213 @default.
• W2017698526 creator A5033868416 @default.
• W2017698526 date "2004-03-01" @default.
• W2017698526 modified "2023-09-23" @default.
• W2017698526 title "Immune response induced by N-lauroylsarcosine extracted outer-membrane proteins of an isolate of Edwardsiella ictaluri in channel catfish" @default.
• W2017698526 cites W1520725208 @default.
• W2017698526 cites W1593691014 @default.
• W2017698526 cites W1752656673 @default.
• W2017698526 cites W1853222534 @default.
• W2017698526 cites W1904576076 @default.
• W2017698526 cites W1925617139 @default.
• W2017698526 cites W1940749008 @default.
• W2017698526 cites W1943943023 @default.
• W2017698526 cites W1945621958 @default.
• W2017698526 cites W1975975446 @default.
• W2017698526 cites W1987066267 @default.
• W2017698526 cites W1997227296 @default.
• W2017698526 cites W2004454844 @default.
• W2017698526 cites W2014139648 @default.
• W2017698526 cites W2015549544 @default.
• W2017698526 cites W2018053316 @default.
• W2017698526 cites W2034162808 @default.
• W2017698526 cites W2043773380 @default.
• W2017698526 cites W2046524869 @default.
• W2017698526 cites W2047283421 @default.
• W2017698526 cites W2051455093 @default.
• W2017698526 cites W2054801018 @default.
• W2017698526 cites W2071395660 @default.
• W2017698526 cites W2077684107 @default.
• W2017698526 cites W2079944549 @default.
• W2017698526 cites W2084880502 @default.
• W2017698526 cites W2088115627 @default.
• W2017698526 cites W2088401193 @default.
• W2017698526 cites W2090367376 @default.
• W2017698526 cites W2094429621 @default.
• W2017698526 cites W2099434149 @default.
• W2017698526 cites W2108540658 @default.
• W2017698526 cites W2114523524 @default.
• W2017698526 cites W2135723813 @default.
• W2017698526 cites W2143459412 @default.
• W2017698526 cites W2144860935 @default.
• W2017698526 cites W2168997585 @default.
• W2017698526 cites W2174423842 @default.
• W2017698526 cites W2235330381 @default.
• W2017698526 cites W2335756740 @default.
• W2017698526 cites W2405167152 @default.
• W2017698526 cites W24748866 @default.
• W2017698526 cites W296453179 @default.
• W2017698526 doi "https://doi.org/10.1016/j.fsi.2003.07.003" @default.
• W2017698526 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15123308" @default.
• W2017698526 hasPublicationYear "2004" @default.
• W2017698526 type Work @default.
• W2017698526 sameAs 2017698526 @default.
• W2017698526 citedByCount "34" @default.
• W2017698526 countsByYear W20176985262012 @default.
• W2017698526 countsByYear W20176985262013 @default.
• W2017698526 countsByYear W20176985262014 @default.
• W2017698526 countsByYear W20176985262015 @default.
• W2017698526 countsByYear W20176985262016 @default.
• W2017698526 countsByYear W20176985262017 @default.
• W2017698526 countsByYear W20176985262019 @default.
• W2017698526 countsByYear W20176985262020 @default.
• W2017698526 countsByYear W20176985262021 @default.
• W2017698526 countsByYear W20176985262022 @default.
• W2017698526 countsByYear W20176985262023 @default.
• W2017698526 crossrefType "journal-article" @default.
• W2017698526 hasAuthorship W2017698526A5021887441 @default.
• W2017698526 hasAuthorship W2017698526A5024503213 @default.
• W2017698526 hasAuthorship W2017698526A5033868416 @default.
• W2017698526 hasConcept C101660505 @default.
• W2017698526 hasConcept C104317684 @default.
• W2017698526 hasConcept C116084860 @default.
• W2017698526 hasConcept C146587185 @default.
• W2017698526 hasConcept C153911025 @default.
• W2017698526 hasConcept C181199279 @default.
• W2017698526 hasConcept C203014093 @default.
• W2017698526 hasConcept C2776415932 @default.
• W2017698526 hasConcept C2776832872 @default.
• W2017698526 hasConcept C2778189562 @default.
• W2017698526 hasConcept C2778754761 @default.
• W2017698526 hasConcept C2778949639 @default.
• W2017698526 hasConcept C2779715253 @default.
• W2017698526 hasConcept C2780868878 @default.
• W2017698526 hasConcept C2909208804 @default.
• W2017698526 hasConcept C2911177997 @default.
• W2017698526 hasConcept C505870484 @default.
• W2017698526 hasConcept C547475151 @default.
• W2017698526 hasConcept C55493867 @default.
• W2017698526 hasConcept C57409179 @default.
• W2017698526 hasConcept C85265743 @default.
• W2017698526 hasConcept C86803240 @default.
• W2017698526 hasConcept C8891405 @default.
• W2017698526 hasConcept C89423630 @default.
• W2017698526 hasConceptScore W2017698526C101660505 @default.
• W2017698526 hasConceptScore W2017698526C104317684 @default.

• next