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• W2017704759 abstract "No AccessJournal of UrologyINVESTIGATIVE UROLOGY1 Sep 2001SPONTANEOUS CA2+ ACTIVATED CL− CURRENTS IN ISOLATED URETHRAL SMOOTH MUSCLE CELLS G.P. SERGEANT, M.A. HOLLYWOOD, N.G. MCHALE, and K.D. THORNBURY G.P. SERGEANTG.P. SERGEANT More articles by this author , M.A. HOLLYWOODM.A. HOLLYWOOD More articles by this author , N.G. MCHALEN.G. MCHALE More articles by this author , and K.D. THORNBURYK.D. THORNBURY More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(05)65939-3AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: We identified and characterized the membrane currents underlying spontaneous transient depolarization in the urethra. Materials and Methods: Myocytes were isolated from sheep urethra by enzymatic digestion and studied by the amphotericin B patch clamp method. Results: Just more than 10% of cells had spontaneous transient inward currents when maintained at −60 mV. Mean amplitude plus or minus standard error of mean of the spontaneous transient inward currents was 102 ± 35 pA. and mean frequency was 17 ± 3 minutes−1 in 18 preparations. Within each cell currents sometimes consisted of up to 3 phases but in 16 of 18 cells monophasic spontaneous transient inward currents were also identified. These currents decayed relatively slowly with a mean time constant of 570 ± 97 ms. Spontaneous transient inward currents were identified as Ca2+ activated Cl− currents because they reversed near the calculated Nernst potential for chloride ions. They were blocked by the Cl− channel blockers 100 μM. niflumic acid and 1 mM. anthracene-9-carboxylic acid as well as in Ca2+-free solution, 10 mM. caffeine and 30 μM. ryanodine. The latter results suggest that spontaneous transient inward currents require intact intracellular Ca2+ stores. Amplitude and frequency were unaffected by 10 μM. nifedipine but were reduced by the nonspecific Ca2+ entry blockers 10 μM. SKF 96365 and 1 mM. La3+. We interpret these results as indicating that the Ca2+ stores underlying the spontaneous transient inward currents may refill by plasmalemmal Ca2+ channels that differ from L-type channels. Conclusions: Urethral cells fire large spontaneous transient inward currents, mediated by Ca2+ activated Cl− channels, which are adequate to account for the spontaneous transient depolarizations seen in whole urethral tissue. References 1 : Nervous control of micturition. Physiol Rev1965; 45: 425. Google Scholar 2 : Relative contributions of smooth and striated muscles to the canine urethra pressure profile. Br J Urol1976; 48: 347. Google Scholar 3 : The physiology of the mammalian urinary outflow tract. Exp Physiol1999; 84: 215. 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Google Scholar 11 : Specialised pacemaking cells in the rabbit urethra. J Physiol2000; 526: 359. Google Scholar 12 : Low access resistance perforated patch recordings using amphotericin-B. J Neurosci Methods1991; 37: 15. Google Scholar 13 : Muscarinic activation of ionic currents measured by a new whole-cell recording method. J Gen Physiol1988; 92: 145. Google Scholar 14 : Correction for liquid junction potentials in patch clamp experiments. Methods Enzymol1992; 207: 123. Google Scholar 15 : Time course of spontaneous calcium-activated chloride currents in smooth muscle cells from the rabbit portal vein. J Physiol1993; 464: 15. Google Scholar 16 : Spontaneous transient inward currents and rhythmicity in canine and guinea pig tracheal smooth muscle cells. Pflugers Arch1994; 427: 473. Google Scholar 17 : Characteristics and physiological role of the Ca2+-activated Cl− conductance in smooth muscle. Am J Physiol1996; 271: C435. Google Scholar 18 : Effects of Cl− channel blockers on Ca-activated chloride and potassium currents in smooth muscle cells from rabbit portal vein. Br J Pharmacol1994; 111: 1333. Google Scholar 19 : Calcium sparks in smooth muscle. Am J Physiol Cell Physiol2000; 278: C235. Google Scholar 20 : Modulation of Ca2+-activated Cl-currents in sheep urethral smooth muscle cells. J Physiol1998; 513P: 95P. Google Scholar 21 : Ca2+ sparks activate K+ and Cl− channels, resulting in spontaneous transient currents in guinea-pig tracheal myocytes. J Physiol1998; 513: 711. Google Scholar 22 : Inactivation of calcium-activated chloride channels in smooth muscle by calcium/calmodulin-dependent protein kinase. Proc Natl Acad Sci USA1997; 94: 14918. Google Scholar 23 : Two distinct membrane currents activated by cyclopiazonic acid-induced calcium store depletion in single smooth muscle cells of the mouse anococcygeus. Br J Pharmacol1996; 117: 566. Google Scholar From the Smooth Muscle Group, Department of Physiology, Queen’s University of Belfast, Belfast, Northern Ireland, United Kingdom© 2001 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited byMALMQVIST U, HEDLUND P, SWÄRD K and ANDERSSON K (2018) Female Pig Urethral Tone is Dependent on Rho Guanosine Triphosphatases and Rho-Associated KinaseJournal of Urology, VOL. 171, NO. 5, (1955-1958), Online publication date: 1-May-2004. Volume 166Issue 3September 2001Page: 1161-1166 Advertisement Copyright & Permissions© 2001 by American Urological Association, Inc.Keywordschloride channelssheepurethramuscle, smoothMetricsAuthor Information G.P. SERGEANT More articles by this author M.A. HOLLYWOOD More articles by this author N.G. MCHALE More articles by this author K.D. THORNBURY More articles by this author Expand All Advertisement PDF downloadLoading ..." @default.
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• W2017704759 title "SPONTANEOUS CA ²⁺ ACTIVATED CL ⁻ CURRENTS IN ISOLATED URETHRAL SMOOTH MUSCLE CELLS" @default.
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