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• W2017715572 abstract "The specificity/non-specificity of helix-induction in charged homopolymers such as polylysine and polyglutamic acid, at neutral pH, by various alcohols namely 2,2,2-trifluoroethanol (TFE), methanol, ethanol and 1-propanol is studied. It is found that all the alcohols used, non-specifically induce helical conformation at high concentrations. In addition, the effect(s) of TFE on an all β-sheet protein, such cardiotoxin analogue I (CTX I) from the Taiwan Cobra (Naja naja atra) is also studied. Evaluation of the helix propensity in the amino-acid sequence of CTX I using helix-coil algorithm, agadir, shows a total of 1.15% helical content in the protein. In CTX I, helical conformation is found to be induced at high concentrations of TFE (≥70% v/v). Interestingly, upon denaturation and reduction of disulfide bridges in CTX I, helix is found to be induced even at low concentrations of TFE (≥20% v/v). The results of this study hints at the possible influence of native tertiary structural interactions and disulfide bridges in the induction of helix by TFE." @default.
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• W2017715572 date "1997-03-01" @default.
• W2017715572 modified "2023-09-25" @default.
• W2017715572 title "Non-specific helix-induction in charged homopolypeptides by alcohols" @default.
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• W2017715572 doi "https://doi.org/10.1016/s0167-4838(96)00191-4" @default.
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