Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017751181> ?p ?o ?g. }
- W2017751181 endingPage "119" @default.
- W2017751181 startingPage "109" @default.
- W2017751181 abstract "Estrogen receptor-mediated induction of zona radiata (ZR) and vitellogenin (VTG) mRNA and protein in rainbow trout (Oncorhynchus mykiss) was compared to assess their utility as biomarkers for exposure to estrogenic compounds. Partial sequences of rainbow trout ZR and beta-actin were cloned by reverse transcriptase polymerase chain reaction (RT-PCR) using degenerate primers based on conserved regions across a number of species. A 549 bp fragment of the rainbow trout ZR-gene showed a high degree of amino acid sequence identity to that of salmon (77%), winter flounder (64%), carp ZP2 (63%) and medaka (61%) ZR-proteins. The 1020 bp beta-actin fragment was approximately 100% identical to sequences from several species. Real-time PCR was used to quantify the induction of ZR-gene and VTG in rainbow trout liver after in vivo exposure to estradiol-17 beta (E(2)) (0.01, 0.1, 1.0 or 10 mg/kg body weight (bw) fish) or alpha-zearalenol (alpha-ZEA) (0.1, 1.0 or 10 mg/kg bw). Real-time PCR and indirect enzyme-linked immunosorbent assay (ELISA) showed that ZR and VTG were induced in both the liver and the plasma after a single injection of E(2) or alpha-ZEA. ZR was more responsive to low levels of E(2) and alpha-ZEA than VTG, and real-time PCR was shown to be more sensitive than the ELISA. Rainbow trout ZR-gene and proteins provide a sensitive biomarker for assessing estrogenic activity." @default.
- W2017751181 created "2016-06-24" @default.
- W2017751181 creator A5009044499 @default.
- W2017751181 creator A5034203553 @default.
- W2017751181 creator A5084897304 @default.
- W2017751181 creator A5089535971 @default.
- W2017751181 date "2000-12-01" @default.
- W2017751181 modified "2023-10-10" @default.
- W2017751181 title "Quantification of rainbow trout (Oncorhynchus mykiss) zona radiata and vitellogenin mRNA levels using real-time PCR after in vivo treatment with estradiol-17β or α-zearalenol" @default.
- W2017751181 cites W1489447527 @default.
- W2017751181 cites W1976139878 @default.
- W2017751181 cites W1984799048 @default.
- W2017751181 cites W1985690584 @default.
- W2017751181 cites W1988695122 @default.
- W2017751181 cites W1994036926 @default.
- W2017751181 cites W2000007125 @default.
- W2017751181 cites W2006856081 @default.
- W2017751181 cites W2007741978 @default.
- W2017751181 cites W2013011656 @default.
- W2017751181 cites W2017191338 @default.
- W2017751181 cites W2018125818 @default.
- W2017751181 cites W2021126166 @default.
- W2017751181 cites W2023704290 @default.
- W2017751181 cites W2030683237 @default.
- W2017751181 cites W2034678159 @default.
- W2017751181 cites W2037024562 @default.
- W2017751181 cites W2037217912 @default.
- W2017751181 cites W2041556008 @default.
- W2017751181 cites W2045972457 @default.
- W2017751181 cites W2063089960 @default.
- W2017751181 cites W2066101120 @default.
- W2017751181 cites W2070721758 @default.
- W2017751181 cites W2083429647 @default.
- W2017751181 cites W2084586277 @default.
- W2017751181 cites W2087525246 @default.
- W2017751181 cites W2090003962 @default.
- W2017751181 cites W2090686533 @default.
- W2017751181 cites W2092195243 @default.
- W2017751181 cites W2092920707 @default.
- W2017751181 cites W2147061855 @default.
- W2017751181 cites W2151859980 @default.
- W2017751181 cites W2152806277 @default.
- W2017751181 cites W2163667209 @default.
- W2017751181 cites W2165139205 @default.
- W2017751181 cites W2170102636 @default.
- W2017751181 cites W2902914441 @default.
- W2017751181 cites W2917452732 @default.
- W2017751181 cites W4254568758 @default.
- W2017751181 cites W4294216491 @default.
- W2017751181 doi "https://doi.org/10.1016/s0960-0760(00)00165-5" @default.
- W2017751181 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11226827" @default.
- W2017751181 hasPublicationYear "2000" @default.
- W2017751181 type Work @default.
- W2017751181 sameAs 2017751181 @default.
- W2017751181 citedByCount "103" @default.
- W2017751181 countsByYear W20177511812012 @default.
- W2017751181 countsByYear W20177511812013 @default.
- W2017751181 countsByYear W20177511812014 @default.
- W2017751181 countsByYear W20177511812015 @default.
- W2017751181 countsByYear W20177511812016 @default.
- W2017751181 countsByYear W20177511812017 @default.
- W2017751181 countsByYear W20177511812018 @default.
- W2017751181 countsByYear W20177511812019 @default.
- W2017751181 countsByYear W20177511812020 @default.
- W2017751181 countsByYear W20177511812021 @default.
- W2017751181 countsByYear W20177511812022 @default.
- W2017751181 crossrefType "journal-article" @default.
- W2017751181 hasAuthorship W2017751181A5009044499 @default.
- W2017751181 hasAuthorship W2017751181A5034203553 @default.
- W2017751181 hasAuthorship W2017751181A5084897304 @default.
- W2017751181 hasAuthorship W2017751181A5089535971 @default.
- W2017751181 hasConcept C104317684 @default.
- W2017751181 hasConcept C153911025 @default.
- W2017751181 hasConcept C207001950 @default.
- W2017751181 hasConcept C2776367567 @default.
- W2017751181 hasConcept C2779078696 @default.
- W2017751181 hasConcept C2779363728 @default.
- W2017751181 hasConcept C2780716708 @default.
- W2017751181 hasConcept C2909208804 @default.
- W2017751181 hasConcept C2994167347 @default.
- W2017751181 hasConcept C48023723 @default.
- W2017751181 hasConcept C505870484 @default.
- W2017751181 hasConcept C54355233 @default.
- W2017751181 hasConcept C55493867 @default.
- W2017751181 hasConcept C86803240 @default.
- W2017751181 hasConceptScore W2017751181C104317684 @default.
- W2017751181 hasConceptScore W2017751181C153911025 @default.
- W2017751181 hasConceptScore W2017751181C207001950 @default.
- W2017751181 hasConceptScore W2017751181C2776367567 @default.
- W2017751181 hasConceptScore W2017751181C2779078696 @default.
- W2017751181 hasConceptScore W2017751181C2779363728 @default.
- W2017751181 hasConceptScore W2017751181C2780716708 @default.
- W2017751181 hasConceptScore W2017751181C2909208804 @default.
- W2017751181 hasConceptScore W2017751181C2994167347 @default.
- W2017751181 hasConceptScore W2017751181C48023723 @default.
- W2017751181 hasConceptScore W2017751181C505870484 @default.
- W2017751181 hasConceptScore W2017751181C54355233 @default.
- W2017751181 hasConceptScore W2017751181C55493867 @default.