Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017752945> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2017752945 endingPage "365" @default.
- W2017752945 startingPage "357" @default.
- W2017752945 abstract "It has been observed in the chick myelin that a protein with a molecular weight of 19.5K, in addition to the major species of myelin basic protein (MBP) (MBP1, mol. wt 18.5K), reacts with anti-MBP serum directed against MBP1. The larger MBP (MBP2 mol. wt 19.5K), which seemingly does not correspond to any of the MBP species from other animal sources, is detected at high level in chick myelin from the initial stage of myelination. MBP1 is easily extracted from the fluffy layer of Folch's upper layer, while MBP2 is extracted by sonication of the acidified fluffy layer. Both extracted MBPs can be purified by using high performance liquid chromatography (HPLC) with a reverse phased column after Sephadex G-100 and CM cellulose column chromatographies. The amino acid compositions of both MBPs are similar to each other. Further, both MBPs are phosphorylated using protein kinase C with 5.3 and 3.6 mol of incorporated phosphates to 1 mol of MBP1 and MBP2, respectively. One dimensional peptide mapping of both MBPs with Staphylococcus aureus V8 protease, carboxypeptidase Y and 2-(2-nitrophenylsulfenyl)-3-methyl-3′-bromoindolenine reveals different degradation patterns. This difference is also demonstrated by cyanogen bromide fragmentation. The MBP2 cleavage has a low degradation rate in contrast to the complete cleavage of MBP1. The two fragments (mol. wt 8 and 9K) from MBP1 and one fragment (mol. wt 7K) from MBP2, which are purified by HPLC, can be sequenced from the N-terminus. It is suggested that the methionine site is different in both MBP1 and MBP2, and there are a small number of amino acid substitutions between both MBPs." @default.
- W2017752945 created "2016-06-24" @default.
- W2017752945 creator A5017283439 @default.
- W2017752945 creator A5058183844 @default.
- W2017752945 creator A5090832580 @default.
- W2017752945 date "1991-01-01" @default.
- W2017752945 modified "2023-09-23" @default.
- W2017752945 title "Presence of another species of myelin basic protein (MBP) in the developing chick brain" @default.
- W2017752945 cites W1486602990 @default.
- W2017752945 cites W1528852151 @default.
- W2017752945 cites W1659613485 @default.
- W2017752945 cites W1966768757 @default.
- W2017752945 cites W1998646238 @default.
- W2017752945 cites W2007877410 @default.
- W2017752945 cites W2027429583 @default.
- W2017752945 cites W2061442956 @default.
- W2017752945 cites W2079588047 @default.
- W2017752945 cites W2082979463 @default.
- W2017752945 cites W2087062084 @default.
- W2017752945 cites W2100837269 @default.
- W2017752945 cites W2119216843 @default.
- W2017752945 cites W2140365038 @default.
- W2017752945 cites W2166511889 @default.
- W2017752945 cites W2168526937 @default.
- W2017752945 doi "https://doi.org/10.1016/0197-0186(91)90167-c" @default.
- W2017752945 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20504712" @default.
- W2017752945 hasPublicationYear "1991" @default.
- W2017752945 type Work @default.
- W2017752945 sameAs 2017752945 @default.
- W2017752945 citedByCount "3" @default.
- W2017752945 crossrefType "journal-article" @default.
- W2017752945 hasAuthorship W2017752945A5017283439 @default.
- W2017752945 hasAuthorship W2017752945A5058183844 @default.
- W2017752945 hasAuthorship W2017752945A5090832580 @default.
- W2017752945 hasConcept C104317684 @default.
- W2017752945 hasConcept C151730666 @default.
- W2017752945 hasConcept C167625842 @default.
- W2017752945 hasConcept C169760540 @default.
- W2017752945 hasConcept C175156509 @default.
- W2017752945 hasConcept C181199279 @default.
- W2017752945 hasConcept C185592680 @default.
- W2017752945 hasConcept C2776714187 @default.
- W2017752945 hasConcept C2777093645 @default.
- W2017752945 hasConcept C2778609137 @default.
- W2017752945 hasConcept C2779281246 @default.
- W2017752945 hasConcept C2780876595 @default.
- W2017752945 hasConcept C43369102 @default.
- W2017752945 hasConcept C43617362 @default.
- W2017752945 hasConcept C529278444 @default.
- W2017752945 hasConcept C55493867 @default.
- W2017752945 hasConcept C86803240 @default.
- W2017752945 hasConceptScore W2017752945C104317684 @default.
- W2017752945 hasConceptScore W2017752945C151730666 @default.
- W2017752945 hasConceptScore W2017752945C167625842 @default.
- W2017752945 hasConceptScore W2017752945C169760540 @default.
- W2017752945 hasConceptScore W2017752945C175156509 @default.
- W2017752945 hasConceptScore W2017752945C181199279 @default.
- W2017752945 hasConceptScore W2017752945C185592680 @default.
- W2017752945 hasConceptScore W2017752945C2776714187 @default.
- W2017752945 hasConceptScore W2017752945C2777093645 @default.
- W2017752945 hasConceptScore W2017752945C2778609137 @default.
- W2017752945 hasConceptScore W2017752945C2779281246 @default.
- W2017752945 hasConceptScore W2017752945C2780876595 @default.
- W2017752945 hasConceptScore W2017752945C43369102 @default.
- W2017752945 hasConceptScore W2017752945C43617362 @default.
- W2017752945 hasConceptScore W2017752945C529278444 @default.
- W2017752945 hasConceptScore W2017752945C55493867 @default.
- W2017752945 hasConceptScore W2017752945C86803240 @default.
- W2017752945 hasIssue "3" @default.
- W2017752945 hasLocation W20177529451 @default.
- W2017752945 hasLocation W20177529452 @default.
- W2017752945 hasOpenAccess W2017752945 @default.
- W2017752945 hasPrimaryLocation W20177529451 @default.
- W2017752945 hasRelatedWork W1527239966 @default.
- W2017752945 hasRelatedWork W1996456151 @default.
- W2017752945 hasRelatedWork W2007717937 @default.
- W2017752945 hasRelatedWork W2014401062 @default.
- W2017752945 hasRelatedWork W2026137340 @default.
- W2017752945 hasRelatedWork W2040972554 @default.
- W2017752945 hasRelatedWork W2063146970 @default.
- W2017752945 hasRelatedWork W2066971713 @default.
- W2017752945 hasRelatedWork W2070044826 @default.
- W2017752945 hasRelatedWork W2160014529 @default.
- W2017752945 hasVolume "18" @default.
- W2017752945 isParatext "false" @default.
- W2017752945 isRetracted "false" @default.
- W2017752945 magId "2017752945" @default.
- W2017752945 workType "article" @default.