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• W2017756612 abstract "Following a promising preliminary study concerning sugar beet leaves valorization and to fulfill the high demand in natural C6-aldehydes flavors, an efficient biocatalytic reaction was developed to synthesize (2E)-hexenal at large scale. As major product of the lipoxygenase enzyme, 13-HPOT was converted by sugar beet hydroperoxide lyase extracted from leaves or expressed by recombinant Escherichia coli strains. With the adaptation of a fed-batch substrate addition and a continuous extraction of volatiles, 3.46 mM and 1.37 mM of C6-aldehydes were produced with the native hydroperoxide lyase at respectively 2 and 100 L scale. Furthermore, higher molar productivity of green leaf volatiles was reached with recombinant hydroperoxide lyase (5.5 mM at 2 L scale) while no others side products from the lipoxygenase pathway were formed. Once purified, these natural aromas are suitable for food and beverage preparations, perfumes or cosmetic products." @default.
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• W2017756612 date "2012-12-01" @default.
• W2017756612 modified "2023-10-13" @default.
• W2017756612 title "Optimization and scaling up of a biotechnological synthesis of natural green leaf volatiles using Beta vulgaris hydroperoxide lyase" @default.
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• W2017756612 doi "https://doi.org/10.1016/j.procbio.2012.07.018" @default.
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