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• W2017756919 abstract "Silk fibroin (SF), isolated from silkworm (Bombyx mori) cocoons, is a natural biodegradable polymer. Over the past decade, there was some interest in using SF as a biomedical material. As part of a project to develop tissue-engineered constructs for the surgical restoration of the ocular surface (cornea, conjunctiva), we have investigated the capacity of SF to function as a substratum for the attachment and growth of corneal stem/progenitor cells harvested from the corneoscleral limbus of donor human corneal tissue. SF membranes were produced from cocoons following a protocol involving successive dissolution steps, filtration, dialysis, evaporation, and methanol treatment. Human limbal epithelial cells were harvested from donor tissue and seeded onto SF membranes. After 5 days, the culture was fixed and stained with specific agents to visualize the cells. The study indicated profuse cellular attachment and growth. SF membranes appear to be suitable as a substratum for the repair of damaged ocular surface." @default.
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• W2017756919 date "2007-10-01" @default.
• W2017756919 modified "2023-10-16" @default.
• W2017756919 title "Silk as Substratum for Cell Attachment and Proliferation" @default.
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• W2017756919 doi "https://doi.org/10.4028/www.scientific.net/msf.561-565.1549" @default.
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