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• W2017796427 abstract "Oligosaccharides are known to play important roles in many biological processes. In the study of oligosaccharides, collision-induced dissociation (CID) is the most common dissociation method to elucidate the sequence and connectivity. However, a disadvantage of CID is the decrease in both the degree and efficiency of dissociation with increasing mass. In the present study, we have successfully performed infrared multiphoton dissociation (IRMPD) on 39 O-linked mucin-type oligosaccharide alditols (both neutral and anionic). CID and IRMPD spectra of several oligosaccharides were also compared. They yielded nearly identical fragment ions corresponding to the lowest energy fragmentation pathways. The characteristic fragmentations of structural motifs, which can provide the linkage information, were similarly presented in both CID and IRMPD spectra. Multistage of CID (MS3 or MS4) is commonly needed to completely sequence the oligosaccharides, while IRMPD of the same compounds yielded the fragment ions corresponding to the loss of the first residue to the last residue during a single-stage tandem MS (MS2). Finally, it is shown that the fragmentation efficiency of IRMPD increases with the increasing size of oligosaccharides." @default.
• W2017796427 created "2016-06-24" @default.
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• W2017796427 date "2004-12-03" @default.
• W2017796427 modified "2023-10-16" @default.
• W2017796427 title "Infrared Multiphoton Dissociation of O-Linked Mucin-Type Oligosaccharides" @default.
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• W2017796427 doi "https://doi.org/10.1021/ac0489824" @default.
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