FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017802952> ?p ?o ?g. }

• W2017802952 endingPage "664" @default.
• W2017802952 startingPage "656" @default.
• W2017802952 abstract "Studies to elucidate the reactions that occur at the eukaryotic replication fork have been limited by the model systems available. We have established a method for isolating and characterizing Simian Virus 40 (SV40) replication complexes. SV40 rolling circle complexes are isolated using paramagnetic beads and then incubated under replication conditions to obtain continued elongation. In rolling circle replication, the normal mechanism for termination of SV40 replication does not occur and the elongation phase of replication is prolonged. Thus, using this assay system, elongation phase reactions can be examined in the absence of initiation or termination. We show that the protein requirements for elongation of SV40 rolling circles are equivalent to complete SV40 replication reactions. The DNA produced by SV40 rolling circles is doublestranded, unmethylated and with a much longer length than the template DNA. These properties are similar to those of physiological replication forks. We show that proteins associated with the isolated rolling circles, including SV40 T antigen, DNA polymerase α, replication protein A (RPA) and RF-C, are necessary for continued DNA synthesis. PCNA is also required but is not associated with the isolated complexes. We present evidence suggesting that synthesis of the leading and lagging strands are co-ordinated in SV40 rolling circle replication. We have used this system to show that both RPA-protein and RPA-DNA interactions are important for RPA's function in elongation." @default.
• W2017802952 created "2016-06-24" @default.
• W2017802952 creator A5039445545 @default.
• W2017802952 creator A5087322488 @default.
• W2017802952 creator A5088674158 @default.
• W2017802952 date "1999-01-01" @default.
• W2017802952 modified "2023-10-10" @default.
• W2017802952 title "A novel assay for examining the molecular reactions at the eukaryotic replication fork: activities of replication protein A required during elongation" @default.
• W2017802952 cites W1493097357 @default.
• W2017802952 cites W1499161907 @default.
• W2017802952 cites W1517952165 @default.
• W2017802952 cites W1517997064 @default.
• W2017802952 cites W1533565768 @default.
• W2017802952 cites W1540214411 @default.
• W2017802952 cites W1550656265 @default.
• W2017802952 cites W1552586455 @default.
• W2017802952 cites W1554850792 @default.
• W2017802952 cites W1558614105 @default.
• W2017802952 cites W1565314684 @default.
• W2017802952 cites W1574197136 @default.
• W2017802952 cites W1580231709 @default.
• W2017802952 cites W1587132435 @default.
• W2017802952 cites W1590134197 @default.
• W2017802952 cites W1605838085 @default.
• W2017802952 cites W1625430625 @default.
• W2017802952 cites W1632041306 @default.
• W2017802952 cites W1640638881 @default.
• W2017802952 cites W1754682352 @default.
• W2017802952 cites W1870805526 @default.
• W2017802952 cites W1971210313 @default.
• W2017802952 cites W1977875386 @default.
• W2017802952 cites W1985206061 @default.
• W2017802952 cites W1988840026 @default.
• W2017802952 cites W1988939915 @default.
• W2017802952 cites W1991547782 @default.
• W2017802952 cites W1992554186 @default.
• W2017802952 cites W1996783278 @default.
• W2017802952 cites W1997454828 @default.
• W2017802952 cites W2003329456 @default.
• W2017802952 cites W2004669805 @default.
• W2017802952 cites W2007910316 @default.
• W2017802952 cites W2008783898 @default.
• W2017802952 cites W2008992593 @default.
• W2017802952 cites W2011083044 @default.
• W2017802952 cites W2013981844 @default.
• W2017802952 cites W2017175281 @default.
• W2017802952 cites W2026933272 @default.
• W2017802952 cites W2031039047 @default.
• W2017802952 cites W2035746057 @default.
• W2017802952 cites W2039584375 @default.
• W2017802952 cites W2039653473 @default.
• W2017802952 cites W2050458733 @default.
• W2017802952 cites W2055348937 @default.
• W2017802952 cites W2057649097 @default.
• W2017802952 cites W2071167503 @default.
• W2017802952 cites W2076867773 @default.
• W2017802952 cites W2080170863 @default.
• W2017802952 cites W2086108718 @default.
• W2017802952 cites W2090110799 @default.
• W2017802952 cites W2102732182 @default.
• W2017802952 cites W2114432362 @default.
• W2017802952 cites W2120139128 @default.
• W2017802952 cites W2122748184 @default.
• W2017802952 cites W2127720955 @default.
• W2017802952 cites W2134327149 @default.
• W2017802952 cites W2134871100 @default.
• W2017802952 cites W2136238750 @default.
• W2017802952 cites W2137982536 @default.
• W2017802952 cites W2138866614 @default.
• W2017802952 cites W2139768549 @default.
• W2017802952 cites W2142485616 @default.
• W2017802952 cites W2142844417 @default.
• W2017802952 cites W2161596679 @default.
• W2017802952 cites W2162424511 @default.
• W2017802952 cites W2166103784 @default.
• W2017802952 cites W2169154093 @default.
• W2017802952 cites W2171775098 @default.
• W2017802952 cites W2185952256 @default.
• W2017802952 cites W4256590580 @default.
• W2017802952 cites W838635636 @default.
• W2017802952 doi "https://doi.org/10.1093/nar/27.2.656" @default.
• W2017802952 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/148229" @default.
• W2017802952 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9862994" @default.
• W2017802952 hasPublicationYear "1999" @default.
• W2017802952 type Work @default.
• W2017802952 sameAs 2017802952 @default.
• W2017802952 citedByCount "21" @default.
• W2017802952 countsByYear W20178029522013 @default.
• W2017802952 crossrefType "journal-article" @default.
• W2017802952 hasAuthorship W2017802952A5039445545 @default.
• W2017802952 hasAuthorship W2017802952A5087322488 @default.
• W2017802952 hasAuthorship W2017802952A5088674158 @default.
• W2017802952 hasBestOaLocation W20178029521 @default.
• W2017802952 hasConcept C104317684 @default.
• W2017802952 hasConcept C104690221 @default.
• W2017802952 hasConcept C112950240 @default.
• W2017802952 hasConcept C113271649 @default.
• W2017802952 hasConcept C12590798 @default.

• next