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- W2017830645 abstract "Mass isolation of pure mitotic apparatuses (MAs) from sea urchin eggs was achieved using a glycerol/Mg2+/Triton X-100 isolation medium. The Mg ions stabilized the fibrous structures of the spindle and asters, while Triton X-100 favored dispersion of cell membranes. The MAs were stable for at least 1 day at 20 °C as indicated by phase contrast microscopy. The MAs also showed stable birefringence and solubility properties over a period of several hours. Only centrospheres remained intact in 0.4 M KCl-containing isolation medium. The 0.4 M KCl extract contained tubulin as one of its major components. Transfer of isolated MAs to an Mg-free medium caused the otherwise stable MA birefringence to decay upon addition of sulfhydryl-blocking reagents or Ca ions that depolymerize MA microtubules. Furthermore, when Mg ions were omitted from the isolation medium, only unstable MAs were obtained. This method seems to be of great advantage in the preparation of pure MAs in large quantity." @default.
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- W2017830645 title "Mass isolation of mitotic apparatus using a glycerol/Mg2+/Triton X-100 medium" @default.
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- W2017830645 doi "https://doi.org/10.1016/0014-4827(77)90117-3" @default.
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