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- W2017841885 abstract "Summary. When treating histidine solutions according to BARSOTTM‐GADDUM‐CODB'S histamine extraction method it has been found that a small part of the histidine will be transformed into histamine. This is in accordance with the findings of ÅKERBLOM (1941). A simple method of extracting histamine from blood is described. To one volume of blood two volumes of ethyl alcohol is added together with H 2 SO 4 sufficient to give the mixture a pH of about 4. The mixture is allowed to stand for 1 1 / 2 hour. After filtration and washing of the precipitate with alcohol the total filtrate is evaporated to a small volume in vacuo. After dilution with water and adjusting the pH to neutral the extract is ready for biological testing. Using this extraction method histamine was quantitatively recovered from water solutions of histamine and also from histamine added to horse blood. The amount of histamine in horse blood has been determined both according to CODE'S method and to the method described in this paper. Using CODE'S method a mean value of about 0.18 μg histamine per ml blood (expressed as dihydrochloride) was found whereas the other method gave a mean value of 0.065 μ per ml. Some part of the histamine obtained with CODE'S method may derive from the histidine in hemoglobin, decarboxylated during the extraction procedure. When extracting pure hemoglobin solutions according to CODE'S method the final extracts contain histamine in a concentration which corresponds to the above‐mentioned difference." @default.
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- W2017841885 date "1949-12-01" @default.
- W2017841885 modified "2023-09-27" @default.
- W2017841885 title "Some Remarks on the Determination of Histamine with Special Reference to Horse Blood" @default.
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- W2017841885 doi "https://doi.org/10.1111/j.1748-1716.1949.tb00156.x" @default.
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